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Metabolic engineering of biomass for high energy density: Oilseed-like triacylglycerol yields from plant leaves

Citation

Vanhercke, T and El Tahchy, A and Liu, Q and Zhou, X-R and Shrestha, P and Divi, UK and Ral, J-P and Mansour, MP and Nichols, PD and James, CN and Horn, PJ and Chapman, KD and Beaudoin, F and Ruiz-Lopez, N and Larkin, PJ and de Feyter, RC and Singh, SP and Petrie, JR, Metabolic engineering of biomass for high energy density: Oilseed-like triacylglycerol yields from plant leaves, Plant Biotechnology Journal, 12, (2) pp. 231-239. ISSN 1467-7644 (2014) [Refereed Article]


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Copyright 2013 CSIRO. Licensed under Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) https://creativecommons.org/licenses/by-nc-nd/3.0/

DOI: doi:10.1111/pbi.12131

Abstract

High biomass crops have recently attracted significant attention as an alternative platform for the renewable production of high energy storage lipids such as triacylglycerol (TAG). While TAG typically accumulates in seeds as storage compounds fuelling subsequent germination, levels in vegetative tissues are generally low. Here, we report the accumulation of more than 15% TAG (17.7% total lipids) by dry weight in Nicotiana tabacum (tobacco) leaves by the co-expression of three genes involved in different aspects of TAG production without severely impacting plant development. These yields far exceed the levels found in wild-type leaf tissue as well as previously reported engineered TAG yields in vegetative tissues of Arabidopsis thaliana and N. tabacum. When translated to a high biomass crop, the current levels would translate to an oil yield per hectare that exceeds those of most cultivated oilseed crops. Confocal fluorescence microscopy and mass spectrometry imaging confirmed the accumulation of TAG within leaf mesophyll cells. In addition, we explored the applicability of several existing oil-processing methods using fresh leaf tissue. Our results demonstrate the technical feasibility of a vegetative plant oil production platform and provide for a step change in the bioenergy landscape, opening new prospects for sustainable food, high energy forage, biofuel and biomaterial applications.

Item Details

Item Type:Refereed Article
Keywords:DGAT1, leaf, Nicotiana tabacum, oleosin, triacylglycerol, WRI1, Arabidopsis thaliana, Nicotiana tabacum
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial Crops
Objective Field:Plant Extract Crops (e.g. Pyrethrum, Jojoba)
Author:Nichols, PD (Dr Peter Nichols)
ID Code:119452
Year Published:2014
Web of Science® Times Cited:69
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2017-08-01
Last Modified:2017-10-16
Downloads:2 View Download Statistics

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