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Habituation of Sulfobacillus thermosulfidooxidans to 4-nonylphenol in ferrous ion growth medium


Collinson, DM and Usher, KM and Nichols, PD and Watling, HR, Habituation of Sulfobacillus thermosulfidooxidans to 4-nonylphenol in ferrous ion growth medium, Process Biochemistry, 46, (1) pp. 108-115. ISSN 1359-5113 (2011) [Refereed Article]

Copyright Statement

2010 Elsevier

DOI: doi:10.1016/j.procbio.2010.07.025


The aim of this study was to resolve the apparent inconsistency between the prevalence of the moderate thermophile Sulfobacillus thermosulfidooxidans in copper sulfide heaps and the sensitivity of this bacterium to the solvent extraction (SX) reagent used to concentrate and purify the copper in solution prior to metal production. More than 1 year was required to achieve habituation to the most inhibitory component of the SX reagent, 4-nonylphenol (4-NP; 2 mg L-1), when S. thermosulfidooxidans was grown in ferrous ion medium. However, habituation to 4-NP did not confer the ability to tolerate higher 4-NP concentrations and those characteristics acquired by the cells during habituation were rapidly lost when grown for a short period in 4-NP-free medium. The culture habituated to the presence of 2 mg L-1 4-NP exhibited differences in cell morphology, cell ultrastructure and membrane fatty acid composition compared with the untreated culture subjected to a similar subculture regime. It is concluded that, where S. thermosulfidooxidans is prevalent in copper sulfide heaps associated with SX-EW plants, this species has become habituated through continuous long-term exposure to low concentrations of 4-NP derived from entrained SX reagent in the recycled leach solutions.

Item Details

Item Type:Refereed Article
Keywords:4-Nonylphenol, acidophilic bacteria, adaptation, ferrous ion oxidation, 4-Nonylphenol, acidophilic bacteria, adaptation, copper sulfides, fatty acid composition, ferrous ion, ferrous ion oxidation, in-cell, leach solutions, long term exposure
Research Division:Earth Sciences
Research Group:Geochemistry
Research Field:Inorganic geochemistry
Objective Division:Mineral Resources (Excl. Energy Resources)
Objective Group:Other mineral resources (excl. energy resources)
Objective Field:Other mineral resources (excl. energy resources) not elsewhere classified
UTAS Author:Nichols, PD (Dr Peter Nichols)
ID Code:119404
Year Published:2011
Web of Science® Times Cited:3
Deposited By:Geography and Spatial Science
Deposited On:2017-08-01
Last Modified:2017-10-16

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