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A bedside assay to detect streptococcus pneumoniae in children with empyema


Strachan, RE and Cornelius, A and Gilbert, GL and Gulliver, T and Martin, A and McDonald, T and Nixon, GM and Roseby, R and Ranganathan, S and Selvadurai, H and Smith, G and Soto-Martinez, M and Suresh, S and Teoh, L and Thapa, K and Wainwright, CE and Jaffe, A, A bedside assay to detect streptococcus pneumoniae in children with empyema, Pediatric Pulmonology, 46, (2) pp. 179-183. ISSN 8755-6863 (2011) [Refereed Article]

Copyright Statement

Copyright 2011 Wiley-Liss

DOI: doi:10.1002/ppul.21349


Background:Empyema is a complication of pneumonia, commonly caused by Streptococcus pneumoniae. Aims To validate the utility of an immunochromatographic test for the detection of S. pneumoniae antigen in the pleural fluid of children with empyema.

Methods: Empyema patients had blood and pleural fluid cultured, and polymerase chain reaction (PCR) to detect the S. pneumoniae autolysin gene, lytA, in pleural fluid. Pleural fluid was tested using the Binax NOW S. pneumoniae antigen detection assay and compared with lytA PCR results and/or culture in blood or pleural fluid.

Results: S. pneumoniae was detected by PCR in pleural fluid of 68 of 137 (49.6%) patients, by culture in 11 of 135 (8.1%) pleural specimens and 16 of 120 (13.3%) blood specimens. Pleural fluid Binax NOW testing from 130 patients demonstrated a sensitivity of 83.8% and specificity of 93.5% (positive predictive value of 93.4% and negative predictive value of 84.1%).

Conclusions: In pediatric empyema, high predictive values of pleural fluid Binax NOW S. pneumoniae antigen test suggest that this test may help rationalize antibiotic choice in these patients.

Item Details

Item Type:Refereed Article
Keywords:Streptococcus pneumoniae, pleural empyema, pediatric, polymerase chain reaction
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Proteomics and intermolecular interactions (excl. medical proteomics)
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Cornelius, A (Dr Anita Cornelius)
ID Code:119321
Year Published:2011
Web of Science® Times Cited:16
Deposited By:Medicine
Deposited On:2017-07-31
Last Modified:2017-09-01

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