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Using time-resolved fluorescence to measure serum venom-specific IgE and IgG


van Eeden, PE and Wiese, MD and Aulfrey, S and Hales, BJ and Stone, SF and Brown, SG, Using time-resolved fluorescence to measure serum venom-specific IgE and IgG, PLoS ONE, 6, (1) pp. 1-7. ISSN 1932-6203 (2011) [Refereed Article]


Copyright Statement

Copyright 2011 van Eeden et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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DOI: doi:10.1371/journal.pone.0016741


We adapted DELFIA™ (dissociation-enhanced lanthanide fluoroimmunoassay), a time resolved fluorescence method, to quantitate whole venom specific and allergenic peptide-specific IgE (sIgE), sIgG1 and sIgG4 in serum from people clinically allergic to Australian native ant venoms, of which the predominant cause of allergy is jack jumper ant venom (JJAV). Intraassay CV was 6.3% and inter-assay CV was 13.7% for JJAV sIgE. DELFIA and Phadia CAP JJAV sIgE results correlated well and had similar sensitivity and specificity for the detection of JJAV sIgE against intradermal skin testing as the gold standard. DELFIA was easily adapted for detecting sIgE to a panel of other native ant venoms. © 2011 van Eeden et al.

Item Details

Item Type:Refereed Article
Keywords:ant venom, immunoglobulin E, immunoglobulin G, allergen, ant venom, immunoglobulin E, immunoglobulin G
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Allergy
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Wiese, MD (Associate Professor Michael Wiese)
UTAS Author:Brown, SG (Professor Simon Brown)
ID Code:119311
Year Published:2011
Web of Science® Times Cited:8
Deposited By:Medicine
Deposited On:2017-07-31
Last Modified:2017-08-02
Downloads:138 View Download Statistics

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