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A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments

Citation

Danilova, OV and Suzina, NE and Van de Camp, J and Svenning, MM and Bodrossy, L and Dedysh, SN, A new cell morphotype among methane oxidizers: a spiral-shaped obligately microaerophilic methanotroph from northern low-oxygen environments, ISME Journal, 10, (11) pp. 2734-2743. ISSN 1751-7362 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 1nterational Society for Microbial Ecology. All rights reserved.

DOI: doi:10.1038/ismej.2016.48

Abstract

Although representatives with spiral-shaped cells are described for many functional groups of bacteria, this cell morphotype has never been observed among methanotrophs. Here, we show that spiral-shaped methanotrophic bacteria do exist in nature but elude isolation by conventional approaches due to the preference for growth under micro-oxic conditions. The helical cell shape may enable rapid motility of these bacteria in water-saturated, heterogeneous environments with high microbial biofilm content, therefore offering an advantage of fast cell positioning under desired high methane/low oxygen conditions. The pmoA genes encoding a subunit of particulate methane monooxygenase from these methanotrophs form a new genus-level lineage within the family Methylococcaceae, type Ib methanotrophs. Application of a pmoA-based microarray detected these bacteria in a variety of high-latitude freshwater environments including wetlands and lake sediments. As revealed by the environmental pmoA distribution analysis, type Ib methanotrophs tend to live very near the methane source, where oxygen is scarce. The former perception of type Ib methanotrophs as being typical for thermal habitats appears to be incorrect because only a minor proportion of pmoA sequences from these bacteria originated from environments with elevated temperatures.

Item Details

Item Type:Refereed Article
Keywords:Methylococcaceae, bacteria
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:119309
Year Published:2016
Web of Science® Times Cited:5
Deposited By:Centre for Ecology and Biodiversity
Deposited On:2017-07-31
Last Modified:2017-10-20
Downloads:0

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