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Polycyclovorans algicola gen. nov., sp. nov., an aromatic-hydrocarbon- degrading marine bacterium found associated with laboratory cultures of marine phytoplankton

Citation

Gutierrez, T and Green, DH and Nichols, PD and Whitman, WB and Semple, KT and Aitken, MD, Polycyclovorans algicola gen. nov., sp. nov., an aromatic-hydrocarbon- degrading marine bacterium found associated with laboratory cultures of marine phytoplankton, Applied and Environmental Microbiology, 79, (1) pp. 205-214. ISSN 0099-2240 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 American Society for Microbiology

DOI: doi:10.1128/AEM.02833-12

Abstract

A strictly aerobic, halotolerant, rod-shaped bacterium, designated strain TG408, was isolated from a laboratory culture of the marine diatom Skeletonema costatum (CCAP1077/1C) by enrichment with polycyclic aromatic hydrocarbons (PAHs) as the sole carbon source. 16S rRNA gene sequence analysis placed this organism within the order Xanthomonadales of the class Gammaproteobacteria. Its closest relatives included representatives of the Hydrocarboniphaga-Nevskia-Sinobacter clade (<92% sequence similarity) in the family Sinobacteraceae. The strain exhibited a narrow nutritional spectrum, preferring to utilize aliphatic and aromatic hydrocarbon compounds and small organic acids. Notably, it displayed versatility in degrading two- and three-ring PAHs. Moreover, catechol 2,3-dioxygenase activity was detected in lysates, indicating that this strain utilizes the metacleavage pathway for aromatic compound degradation. Cells produced surface blebs and contained a single polar flagellum. The predominant isoprenoid quinone of strain TG408 was Q-8, and the dominant fatty acids were C16:0, C16:1 ω7c, and C18:1 ω7c. The G+C content of the isolate's DNA was 64.3 molą0.34 mol%. On the basis of distinct phenotypic and genotypic characteristics, strain TG408 represents a novel genus and species in the class Gammaproteobacteria for which the name Polycyclovorans algicola gen. nov., sp. nov., is proposed. Quantitative PCR primers targeting the 16S rRNA gene of this strain were developed and used to show that this organism is found associated with other species of marine phytoplankton. Phytoplankton may be a natural biotope in the ocean where new species of hydrocarbon-degrading bacteria await discovery and which contribute significantly to natural remediation processes.

Item Details

Item Type:Refereed Article
Keywords:16S rRNA gene, 16S rRNA gene sequence, Gammaproteobacteria, halotolerant, hydrocarbon compounds, isoprenoids, lysates, marine bacterium, marine diatoms, marine phytoplankton, meta-cleavage pathway, new species, polycyclic aromatic hydrocarbons (PAHs)
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and Estuarine Ecology (incl. Marine Ichthyology)
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Marine Flora, Fauna and Biodiversity
Author:Nichols, PD (Dr Peter Nichols)
ID Code:118965
Year Published:2013
Web of Science® Times Cited:22
Deposited By:Zoology
Deposited On:2017-07-24
Last Modified:2017-10-16
Downloads:0

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