'Solid state' electrophoresis

A new approach of electrophoresis on a solvent-less solid state platform, namely Polymer Inclusion Membrane (PIMs), was demonstrated. The transparent, 15 microns thick membrane was composed of Cellulose Triacetate (CTA) polymer, 2-Nitrophenyl Octyl Ether (NPOE) plasticizer and ionic liquid 1-Ethyl-3-methylimidazoleium bis(trifluoromethylsulfonyl)imide [EMIM][NTf₂] and was easily fabricated by dissolving in dichloromethane and drop-casted on glass petri dish. Optimization of membrane chemical compositions and successful separation· of three positively charged fluorescence dyes were reported under 2 kV for 60 min and was monitored using a blue light portable fluorescence microscope cameras.