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Chapter 18. Morphological Analysis of the Zebrafish Digestive System

Citation

Trotter, AJ and Parslow, AC and Heath, JK, Chapter 18. Morphological Analysis of the Zebrafish Digestive System, Zebrafish: Methods and Protocols, Humana Press, GJ Lieschke, AC Oates, K Kawakami (ed), pp. 289-315. ISBN 978-1-60327-976-5 (2009) [Other Book Chapter]


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DOI: doi:10.1007/978-1-60327-977-2_18

Abstract

The zebrafish provides an ideal model for the study of vertebrate organogenesis, including the formation of the digestive tract and its associated organs. Despite optical transparency of embryos, the internal position of the developing digestive system and its close juxtaposition with the yolk initially made morphological analysis relatively challenging, particularly during the first 3 d of development. However, methodologies have been successfully developed to address these problems and comprehensive morphologic analysis of the developing digestive system has now been achieved using a combination of light and fluorescence microscope approaches—including confocal analysis—to visualize wholemount and histological preparations of zebrafish embryos. Furthermore, the expanding number of antibodies that cross-react with zebrafish proteins and the generation of tissue-specific transgenic green fluorescent protein reporter lines that mark specific cell and tissue compartments have greatly enhanced our ability to successfully image the developing zebrafish digestive system.

Item Details

Item Type:Other Book Chapter
Keywords:zebrafish, intestine, epithelium, goblet cells, enteroendocrine cells, histology, immunohistochemistry, transgenic lines
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Biochemistry and Cell Biology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Trotter, AJ (Dr Andrew Trotter)
ID Code:118738
Year Published:2009
Deposited By:IMAS - Directorate
Deposited On:2017-07-19
Last Modified:2017-07-28
Downloads:0

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