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Folate acts in E. coli to accelerate C. elegans aging independently of bacterial biosynthesis


Virk, B and Jia, J and Maynard, CA and Raimundo, A and Lefebvre, J and Richards, S and Chetina, N and Liang, Y and Helliwell, N and Cipinska, M and Weinkove, D, Folate acts in E. coli to accelerate C. elegans aging independently of bacterial biosynthesis, Cell Reports, 14, (7) pp. 1611-1620. ISSN 2211-1247 (2016) [Refereed Article]

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Copyright 2016 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1016/j.celrep.2016.01.051


Folates are cofactors for biosynthetic enzymes in all eukaryotic and prokaryotic cells. Animals cannot synthesize folate and must acquire it from their diet or microbiota. Previously, we showed that inhibiting E. coli folate synthesis increases C. elegans lifespan. Here, we show that restriction or supplementation of C. elegans folate does not influence lifespan. Thus, folate is required in E. coli to shorten worm lifespan. Bacterial proliferation in the intestine has been proposed as a mechanism for the life-shortening influence of E. coli. However, we found no correlation between C. elegans survival and bacterial growth in a screen of 1,000+ E. coli deletion mutants. Nine mutants increased worm lifespan robustly, suggesting specific gene regulation is required for the life-shortening activity of E. coli. Disrupting the biosynthetic folate cycle did not increase lifespan. Thus, folate acts through a growth-independent route in E. coli to accelerate animal aging.

Item Details

Item Type:Refereed Article
Keywords:folate, aging, C. elegans, E. coli
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal physiology - cell
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Richards, S (Dr Shane Richards)
ID Code:118696
Year Published:2016
Web of Science® Times Cited:53
Deposited By:Office of the School of Natural Sciences
Deposited On:2017-07-18
Last Modified:2017-09-22
Downloads:141 View Download Statistics

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