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Characterization of oilseed lipids from “DHA-producing Camelina sativa”: A new transformed land plant containing long-chain omega-3 oils


Mansour, MP and Shrestha, P and Belide, S and Petrie, JR and Nichols, PD and Singh, SP, Characterization of oilseed lipids from 'DHA-producing Camelina sativa': A new transformed land plant containing long-chain omega-3 oils, Nutrients, 6, (2) pp. 776-789. ISSN 2072-6643 (2014) [Refereed Article]


Copyright Statement

© 2014 by the authors; licensee MDPI, Basel, Switzerland. Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0)

DOI: doi:10.3390/nu6020776


New and sustainable sources of long-chain (LC, ≥C20) omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids) and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids) in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (α-linolenic acid, 18:3ω3) in DHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C16–C22 fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0, iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.

Item Details

Item Type:Refereed Article
Keywords:DHA, docosahexaenoic acid, Camelina sativa, omega-3, long-chain PUFA, triacylglycerol, polar lipids, glycolipids, phospholipids, fish oils
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant cell and molecular biology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Nichols, PD (Dr Peter Nichols)
ID Code:118615
Year Published:2014
Web of Science® Times Cited:37
Deposited By:Directorate
Deposited On:2017-07-14
Last Modified:2017-08-09
Downloads:138 View Download Statistics

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