118615 - DHA-producing camelina sativa.pdf (205.96 kB)
Characterization of oilseed lipids from “DHA-producing Camelina sativa”: A new transformed land plant containing long-chain omega-3 oils
journal contribution
posted on 2023-05-19, 07:39 authored by Mansour, MP, Shrestha, P, Belide, S, Petrie, JR, Peter Nichols, Singh, SPNew and sustainable sources of long-chain (LC, ≥C20) omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids) and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids) in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (α-linolenic acid, 18:3ω3) in DHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C16–C22 fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0, iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.
History
Publication title
NutrientsVolume
6Pagination
776-789ISSN
2072-6643Department/School
Institute for Marine and Antarctic StudiesPublisher
M D P I AGPlace of publication
St. Alban-Anlage 66, Basel, 4052 SwitzerlandRights statement
© 2014 by the authors; licensee MDPI, Basel, Switzerland. Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0) http://creativecommons.org/licenses/by/3.0/Repository Status
- Open