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A simple, low-cost platform for real-time isothermal nucleic acid amplification

Citation

Craw, P and Mackay, RE and Naveenathayalan, A and Hudson, C and Branavan, M and Sadiq, ST and Balachandran, W, A simple, low-cost platform for real-time isothermal nucleic acid amplification, Sensors, 15, (9) pp. 23418-23430. ISSN 1424-8220 (2015) [Refereed Article]

DOI: doi:10.3390/s150923418

Abstract

Advances in microfluidics and the introduction of isothermal nucleic acid amplification assays have resulted in a range of solutions for nucleic acid amplification tests suited for point of care and field use. However, miniaturisation of instrumentation for such assays has not seen such rapid advances and fluorescence based assays still depend on complex, bulky and expensive optics such as fluorescence microscopes, photomultiplier tubes and sensitive lens assemblies. In this work we demonstrate a robust, low cost platform for isothermal nucleic acid amplification on a microfluidic device. Using easily obtainable materials and commercial off-the-shelf components, we show real time fluorescence detection using a low cost photodiode and operational amplifier without need for lenses. Temperature regulation on the device is achieved using a heater fabricated with standard printed circuit board fabrication methods. These facile construction methods allow fabrications at a cost compatible with widespread deployment to resource poor settings.

Item Details

Item Type:Refereed Article
Keywords:point-of care, molecular diagnostics, isothermal amplification, nucleic acid amplification test, helicase dependent amplification
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Sensor Technology (Chemical aspects)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Craw, P (Dr Pascal Craw)
ID Code:118583
Year Published:2015
Web of Science® Times Cited:7
Deposited By:Chemistry
Deposited On:2017-07-14
Last Modified:2017-07-14
Downloads:0

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