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A simple, low-cost platform for real-time isothermal nucleic acid amplification
journal contribution
posted on 2023-05-19, 07:36 authored by Pascal Craw, Mackay, RE, Naveenathayalan, A, Hudson, C, Branavan, M, Sadiq, ST, Balachandran, WAdvances in microfluidics and the introduction of isothermal nucleic acid amplification assays have resulted in a range of solutions for nucleic acid amplification tests suited for point of care and field use. However, miniaturisation of instrumentation for such assays has not seen such rapid advances and fluorescence based assays still depend on complex, bulky and expensive optics such as fluorescence microscopes, photomultiplier tubes and sensitive lens assemblies. In this work we demonstrate a robust, low cost platform for isothermal nucleic acid amplification on a microfluidic device. Using easily obtainable materials and commercial off-the-shelf components, we show real time fluorescence detection using a low cost photodiode and operational amplifier without need for lenses. Temperature regulation on the device is achieved using a heater fabricated with standard printed circuit board fabrication methods. These facile construction methods allow fabrications at a cost compatible with widespread deployment to resource poor settings.
History
Publication title
SensorsVolume
15Issue
9Pagination
23418-23430ISSN
1424-8220Department/School
School of Natural SciencesPublisher
Molecular Diversity Preservation InternationalPlace of publication
Matthaeusstrasse 11, Basel, Switzerland, Ch-4057Repository Status
- Restricted