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A novel series of C18C22trans ω3 PUFA from Northern and Southern Hemisphere strains of the marine haptophyte Imantonia rotunda


Lee Chang, KJ and Dunstan, GA and Mansour, MP and Jameson, ID and Nichols, PD, A novel series of C18-C22trans ω3 PUFA from Northern and Southern Hemisphere strains of the marine haptophyte Imantonia rotunda, Journal of Applied Phycology, 28, (6) pp. 3363-3370. ISSN 0921-8971 (2016) [Refereed Article]

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Copyright 2016 Springer Science+Business Media Dordrecht

DOI: doi:10.1007/s10811-016-0906-4


A series of novel C18C22trans ω3 polyunsaturated fatty acids (PUFA) with a single trans double bond in the ω3 position was found in Northern and Southern Hemisphere strains of the marine haptophyte Imantonia rotunda. The novel ω3 PUFA were identified as 18:3(9c,12c,15t) (0.21.8% of total fatty acids), 18:4(6c,9c,12c,15t) (1.94.1%), 18:5 (3c,6c,9c,12c,15t) (0.78.8%), 20:5(5c,8c,11c,14c,17t) (1.24.1%) and 22:6(4c,7c,10c,13c,16c,19t) (0.34.3%), and were accompanied by larger proportions of the all cis isomers: 18:3ω3(9,12,15) (2.73.5%), 18:4ω3(6,9,12,15) (9.314.3%), 18:5ω3(3,6,9,12,15) (7.818.5%), 20:5ω3(5,8,11,14,17) (3.23.9%), 22:5ω3(7,10,13,16,19) (0.10.3%) and 22:6ω3(4,7,10,13,16,19) (2.35.2%). GC analysis of FAME using a non-polar column did not reveal the trans isomers as they coeluted with the all cis PUFA. However, GC using a polar column resolved the trans PUFA from the all cis PUFA, with the trans isomers eluting before the all cis isomers. GC-MS of FAME fractionated by argentation solid-phase chromatography confirmed the molecular ions of all components. FAME were derivatized to form 4,4-dimethyloxazoline (DMOX) derivatives, and GC-MS revealed the same double bond positions for each trans and cis FAME. The results suggest that the ω3 trans double bond originated from the Δ15/ω3 desaturation of 18:2(9c,12c), suggesting that this desaturase has dual cis/trans activity in these species. These results indicate that 18:3(9c,12c,15 t) was the precursor trans isomer produced for the trans series and further desaturation by the common Δ6 desaturase to produce the trans tetraene and successive elongations and desaturations led to the subsequent series of trans ω3 PUFA isomers. To our knowledge, this is the first report of these trans ω3 isomers occurring in strains of I. rotunda. These trans ω3 PUFA may be used as biomarkers in marine food webs for this species and with their unique structure may be biologically active.

Item Details

Item Type:Refereed Article
Keywords:haptophyta, Imantonia, chemotaxonomy, trans PUFA, trans w3 LC-PUFA, trans w3 PUFA, trans delta15 desaturase, trans w3 desaturase, delta15 desaturase
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Phycology (incl. Marine Grasses)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Lee Chang, KJ (Mr Kim Lee Chang)
Author:Jameson, ID (Mr Ian Jameson)
Author:Nichols, PD (Dr Peter Nichols)
ID Code:118285
Year Published:2016
Deposited By:Directorate
Deposited On:2017-07-10
Last Modified:2017-10-25

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