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Using a multi-biomarker approach to assess the effects of pollution on sand flathead (Platycephalus bassensis) from Port Phillip Bay, Victoria, Australia

Citation

Fu, D and Bridle, A and Leef, M and Gagnon, MM and Hassell, KL and Nowak, BF, Using a multi-biomarker approach to assess the effects of pollution on sand flathead (Platycephalus bassensis) from Port Phillip Bay, Victoria, Australia, Marine Pollution Bulletin, 119, (1) pp. 211-219. ISSN 0025-326X (2017) [Refereed Article]

Copyright Statement

2017 Elsevier

DOI: doi:10.1016/j.marpolbul.2017.03.067

Abstract

Hepatic gene expression and liver histology were examined in sand flathead (Platycephalus bassensis) from six locations in Port Phillip Bay, Victoria, Australia. Four sets of genes including thyroid-related genes (D1, D2, TTR, TRα and TRβ), metal metabolism-related genes (MT, MTF1, TF, Ferritin and FPN1), apoptosis-related genes (Diablo/SMAC1, Diablo/SMAC2 and CYP1A) and an endoplasmic reticulum stress biomarker gene (GRP78) were examined in female flathead using qRT-PCR. TRβ and Diablo/SMAC1 gene expression was significantly up-regulated in fish from all polluted sites compared to those from a reference site. The transcripts of TRα and FPN1 were significantly higher in flathead from Corio Bay, while the hepatic mRNA of TTR and GRP78 were significantly lower in those fish. Positive correlations were observed between Diablo/SMAC1 and CYP1A, D2 and TRβ, TRα and TRβ. This study demonstrates that application of pathway-based biomarker genes and histopathology can provide comprehensive information on the impact of environmental pollutants on fish.

Item Details

Item Type:Refereed Article
Keywords:biomarkers, histology, gene expression, sand flathead, Port Phillip Bay
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Pests and Diseases
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Coastal and Estuarine Flora, Fauna and Biodiversity
Author:Fu, D (Mr Dingkun Fu)
Author:Bridle, A (Dr Andrew Bridle)
Author:Leef, M (Dr Melanie Leef)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:118101
Year Published:2017
Web of Science® Times Cited:2
Deposited By:Fisheries and Aquaculture
Deposited On:2017-07-04
Last Modified:2018-03-22
Downloads:0

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