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Rapid and accurate identification by real-time PCR of biotoxin-producing dinoflagellates from the family Gymnodiniaceae

Citation

Smith, KF and de Salas, M and Adamson, J and Rhodes, LL, Rapid and accurate identification by real-time PCR of biotoxin-producing dinoflagellates from the family Gymnodiniaceae, Marine Drugs, 12, (3) pp. 1361-1376. ISSN 1660-3397 (2014) [Refereed Article]


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Copyright 2014 the authors; licensee MDPI, Basel, Switzerland. Licensed under Creative Commons Attribution 3.0 Unported (CC BY 3.0) https://creativecommons.org/licenses/by/3.0/

DOI: doi:10.3390/md12031361

Abstract

The identification of toxin-producing dinoflagellates for monitoring programmes and bio-compound discovery requires considerable taxonomic expertise. It can also be difficult to morphologically differentiate toxic and non-toxic species or strains. Various molecular methods have been used for dinoflagellate identification and detection, and this study describes the development of eight real-time polymerase chain reaction (PCR) assays targeting the large subunit ribosomal RNA (LSU rRNA) gene of species from the genera Gymnodinium, Karenia, Karlodinium, and Takayama. Assays proved to be highly specific and sensitive, and the assay for G. catenatum was further developed for quantification in response to a bloom in Manukau Harbour, New Zealand. The assay estimated cell densities from environmental samples as low as 0.07 cells per PCR reaction, which equated to three cells per litre. This assay not only enabled conclusive species identification but also detected the presence of cells below the limit of detection for light microscopy. This study demonstrates the usefulness of real-time PCR as a sensitive and rapid molecular technique for the detection and quantification of micro-algae from environmental samples.

Item Details

Item Type:Refereed Article
Keywords:biotoxin production, dinoflagellate monitoring, large subunit ribosomal RNA (LSU rRNA) gene, real-time PCR
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Control of Pests, Diseases and Exotic Species
Objective Field:Control of Pests, Diseases and Exotic Species in Marine Environments
Author:de Salas, M (Dr Miguel de Salas)
ID Code:118031
Year Published:2014
Web of Science® Times Cited:10
Deposited By:Plant Science
Deposited On:2017-06-30
Last Modified:2017-09-13
Downloads:0

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