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Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia

Citation

Tang, L and Smith-Keune, C and Gardner, MG and Hardesty, BD, Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia, Conservation Genetics Resources, 6, (1) pp. 143-146. ISSN 1877-7252 (2014) [Refereed Article]

Copyright Statement

Copyright 2013 Springer Science+Business Media Dordrecht

DOI: doi:10.1007/s12686-013-0028-0

Abstract

The Black-throated Finch (Southern) (Poephila cincta cincta) is threatened by the substantial landscape changes in northern Australia. We developed 16 polymorphic microsatellite markers using 454-shotgun whole-genome sequencing technology. We identified an average of 4.7 alleles per locus based on 63 wild caught individuals from Townsville, Queensland. Thirteen and 9 markers were also successfully cross-amplified in two confamilial species, the Double-barred Finch (Taeniopygia bichenovii) and the chestnut-breasted Mannikin (Lonchura castaneothorax) with 11 and 5 were polymorphic, respectively. These markers will help understand the population genetic structure of the endangered Black-throated Finch and determine genetic consequences of landscape changes for the species.

Item Details

Item Type:Refereed Article
Keywords:black-throated finch, conservation genetics, microsatellite, next generation sequencing, Poephila cincta
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Population, Ecological and Evolutionary Genetics
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Flora, Fauna and Biodiversity at Regional or Larger Scales
Author:Hardesty, BD (Dr Britta Hardesty)
ID Code:118019
Year Published:2014
Web of Science® Times Cited:1
Deposited By:Zoology
Deposited On:2017-06-30
Last Modified:2017-10-31
Downloads:0

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