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Water addition regulates the metabolic activity of ammonia oxidizers responding to environmental perturbations in dry subhumid ecosystems

Citation

Hu, HW and MacDonald, CA and Trivedi, P and Holmes, B and Bodrossy, L and He, J-Z and Singh, BK, Water addition regulates the metabolic activity of ammonia oxidizers responding to environmental perturbations in dry subhumid ecosystems, Environmental Microbiology, 17, (2) pp. 444-461. ISSN 1462-2912 (2015) [Refereed Article]

Copyright Statement

Copyright 2014 Society for Applied Microbiology and John Wiley & Sons Ltd

DOI: doi:10.1111/1462-2920.12481

Abstract

Terrestrial arid and semi-arid ecosystems (drylands) constitute about 41% of the Earth's land surface and are predicted to experience increasing fluctuations in water and nitrogen availability. Mounting evidence has confirmed the significant importance of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in nitrification, plant nitrogen availability and atmospheric N2O emissions, but their responses to environmental perturbations in drylands remain largely unknown. Here we evaluate how the factorial combinations of irrigation and fertilization in forests and land-use change from grassland to forest affects the dynamics of AOA and AOB following a 6-year dryland field study. Potential nitrification rates and AOA and AOB abundances were significantly higher in the irrigated plots, accompanied by considerable changes in community compositions, but their responses to fertilization alone were not significant. DNA-stable isotope probing results showed increased 13CO2 incorporation into the amoA gene of AOA, but not of AOB, in plots receiving water addition, coupled with significantly higher net mineralization and nitrification rates. High-throughput microarray analysis revealed that active AOA assemblages belonging to Nitrosopumilus and Nitrosotalea were increasingly labelled by 13CO2 following irrigation. However, no obvious effects of land-use changes on nitrification rates or metabolic activity of AOA and AOB could be observed under dry conditions. We provide evidence that water addition had more important roles than nitrogen fertilization in influencing the autotrophic nitrification in dryland ecosystems, and AOA are increasingly involved in ammonia oxidation when dry soils become wetted.

Item Details

Item Type:Refereed Article
Keywords:ammonia, fertilizer, nitrogen, soil, water
Research Division:Agricultural and Veterinary Sciences
Research Group:Other Agricultural and Veterinary Sciences
Research Field:Fertilisers and Agrochemicals (Application etc.)
Objective Division:Environment
Objective Group:Land and Water Management
Objective Field:Sparseland, Permanent Grassland and Arid Zone Land and Water Management
Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:117873
Year Published:2015
Web of Science® Times Cited:40
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-06-28
Last Modified:2017-09-29
Downloads:0

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