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Prenatal nicotine exposure increases hyperventilation in α4-knock-out mice during mild asphyxia

Citation

Avraam, J and Choen, G and Crago, J and Frappell, P, Prenatal nicotine exposure increases hyperventilation in α4-knock-out mice during mild asphyxia, Respiration Physiology and Neurobiology, 208 pp. 29-36. ISSN 1569-9048 (2015) [Refereed Article]

DOI: doi:10.1016/j.resp.2015.01.006

Abstract

Prenatal nicotine exposure alters breathing and ventilatory responses to stress through stimulation of nicotine acetylcholine receptors (nAChRs). We tested the hypothesis that α4-containing nAChRs are involved in mediating the effects of prenatal nicotine exposure on ventilatory and metabolic responses to intermittent mild asphyxia (MA). Using open-flow plethysmography, we measured ventilation (V˙E) and rate of O2 consumption (V˙O2) of wild-type (WT) and α4-knock-out (KO) mice, at postnatal (P) days 1-2 and 7-8, with and without prenatal nicotine exposure (6mgkg-1day-1 beginning on embryonic day 14). Mice were exposed to seven 2min cycles of mild asphyxia (10% O2 and 5% CO2), each interspersed with 2min of air. Compared to WT, α4 KO mice had increased air V˙E and V˙O2 at P7-8, but not P1-2. Irrespective of age, genotype had no effect on the hyperventilatory response (increase in V˙E/V˙O2) to MA. At P1-2, nicotine suppressed air V˙E and V˙O2 in both genotypes but did not affect the hyperventilatory response to MA. At P7-8 nicotine suppressed air V˙E and V˙O2 of only α4 KO's but also significantly enhanced V˙E during MA (nearly double that of WT; p<0.001). This study has revealed complex effects of α4 nAChR deficiency and prenatal nicotine exposure on ventilatory and metabolic interactions and responses to stress.

Item Details

Item Type:Refereed Article
Keywords:Alpha-4 nicotine receptor subunit, asphyxia, hyperventilation, knock-out mouse, nicotine, ventilation
Research Division:Medical and Health Sciences
Research Group:Paediatrics and Reproductive Medicine
Research Field:Foetal Development and Medicine
Objective Division:Health
Objective Group:Other Health
Objective Field:Health not elsewhere classified
Author:Frappell, P (Professor Peter Frappell)
ID Code:117856
Year Published:2015
Deposited By:Zoology
Deposited On:2017-06-28
Last Modified:2017-11-03
Downloads:0

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