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CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development

Citation

Ventura, T and Bose, U and Fitzgibbon, QP and Smith, GG and Shaw, PN and Cummins, SF and Elizur, A, CYP450s analysis across spiny lobster metamorphosis identifies a long sought missing link in crustacean development, Journal of Steroid Biochemistry and Molecular Biology, 171 pp. 262-269. ISSN 0960-0760 (2017) [Refereed Article]

Copyright Statement

Crown Copyright 2017 Published by Elsevier

DOI: doi:10.1016/j.jsbmb.2017.04.007

Abstract

Cytochrome P450s (CYP450s) are a rapidly evolving family of enzymes, making it difficult to identify bona fide orthologs with notable lineage-specific exceptions. In ecdysozoans, a small number of the most conserved orthologs include enzymes which metabolize ecdysteroids. Ecdysone pathway components were recently shown in a decapod crustacean but with a notable absence of shade, which is important for converting ecdysone to its active form, 20-hydroxyecdysone (20HE), suggesting that another CYP450 performs a similar function in crustaceans. A CYPome temporal expression analysis throughout metamorphosis performed in this research highlights several un-annotated CYP450s displaying differential expression and provides information into expression patterns of annotated CYP450s. Using the expression patterns in the Eastern spiny lobster Sagmariasus verreauxi, followed by 3D modelling and finally activity assays in vitro, we were able to conclude that a group of CYP450s, conserved across decapod crustaceans, function as the insect shade. To emphasize the fact that these genes share the function with shade but are phylogenetically distinct, we name this enzyme system Shed.

Item Details

Item Type:Refereed Article
Keywords:lobster, hormones, metamorphosis, CYP450, ecdysone, molt
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Fisheries - Aquaculture not elsewhere classified
Author:Fitzgibbon, QP (Dr Quinn Fitzgibbon)
Author:Smith, GG (Associate Professor Gregory Smith)
ID Code:117841
Year Published:2017
Funding Support:Australian Research Council (DP160103320)
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2017-06-28
Last Modified:2017-12-13
Downloads:0

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