Physiological and molecular mechanisms mediating xylem Na+ loading in barley in the context of salinity stress tolerance

Time-dependent kinetics of xylem Na⁺ loading was investigated using a large number of barley genotypes contrasting in their salinity tolerance. Salt-sensitive varieties were less efficient in controlling xylem Na⁺ loading and showed a gradual increase in the xylem Na⁺ content over the time. To understand underlying ionic and molecular mechanisms, net fluxes of Ca²⁺, K⁺ and Na⁺ were measured from the xylem parenchyma tissue in response to H₂O₂ and ABA; both of them associated with salinity stress signalling. Our results indicate that NADPH oxidase-mediated apoplastic H₂O₂ production acts upstream of the xylem Na⁺ loading and is causally related to ROS-inducible Ca²⁺ uptake systems in the root stelar tissue. It was also found that ABA regulates (directly or indirectly) the process of Na⁺ retrieval from the xylem and the significant reduction of Na⁺ and K⁺ fluxes induced by bumetanide are indicative of a major role of chloride cation co-transporter (CCC) on xylem ion loading. Transcript levels of HvHKT1;5_like and HvSOS1_like genes in the root stele were observed to decrease after salt stress, while there was an increase in HvSKOR_like gene, indicating that these ion transporters are involved in primary Na⁺/K⁺ movement into/out of xylem.