117476 - increased sucrose in the hypocotyls of radish sprouts.pdf (3.22 MB)
Increased sucrose in the hypocotyls of radish sprouts contributes to nitrogen deficiency-induced anthocyanin accumulation
journal contribution
posted on 2023-05-19, 06:02 authored by Su, N, Wu, Q, Cui, JEffects of nitrogen (N) deficiency and sucrose (Suc) addition on regulation of anthocyanin biosynthesis and their relationship were investigated in this study. Radish sprouts subjected to N deficiency had 50% higher anthocyanin accumulation than when grown in Hoagland solution (a nutrient medium with all macronutrients). The contents of endogenous soluble sugars (Suc, fructose, and glucose) in the hypocotyls were also markedly increased by N limitation, with Suc showing the highest increase. Inhibition of carbohydrate biosynthesis by addition of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) also eliminated N deficiency-induced anthocyanin accumulation. The latter was further supported by the expression of anthocyanin biosynthesis related genes and decreased activities of nitrate reductase in the presence of Suc. Together our results indicate that N deficiency-induced anthocyanin accumulation was, at least partly, dependent on the increase of the soluble sugar, especially Suc. This work is the first comprehensive study on relationship between N deficiency and sugar content on anthocyanin accumulation in the hypocotyls of radish sprouts.
History
Publication title
Frontiers in Plant ScienceVolume
7Article number
1976Number
1976Pagination
1-11ISSN
1664-462XDepartment/School
School of Natural SciencesPublisher
Frontiers Research FoundationPlace of publication
EPFL Science Park, Lausanne, 1015 SwitzerlandRights statement
© 2016 Su, Wu and Cui. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) http://creativecommons.org/licenses/by-nc-nd/4.0/Repository Status
- Open