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Peatland succession induces a shift in the community composition of Sphagnum-associated active methanotrophs

Citation

Putkinen, A and Larmola, T and Tuomivirta, T and Siljanen, HMP and Bodrossy, L and Tuittila, E-S and Fritze, H, Peatland succession induces a shift in the community composition of Sphagnum-associated active methanotrophs, FEMS Microbiology Ecology: (Federation of European Microbiological Societies), 88, (3) pp. 596-611. ISSN 0168-6496 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Federation of European Microbiological Societies

DOI: doi:10.1111/1574-6941.12327

Abstract

Sphagnum-associated methanotrophs (SAM) are an important sink for the methane (CH4) formed in boreal peatlands. We aimed to reveal how peatland succession, which entails a directional change in several environmental variables, affects SAM and their activity. Based on the pmoA microarray results, SAM community structure changes when a peatland develops from a minerotrophic fen to an ombrotrophic bog. Methanotroph subtypes Ia, Ib, and II showed slightly contrasting patterns during succession, suggesting differences in their ecological niche adaptation. Although the direct DNA-based analysis revealed a high diversity of type Ib and II methanotrophs throughout the studied peatland chronosequence, stable isotope probing (SIP) of the pmoA gene indicated they were active mainly during the later stages of succession. In contrast, type Ia methanotrophs showed active CH4 consumption in all analyzed samples. SIP-derived 13C-labeled 16S rRNA gene clone libraries revealed a high diversity of SAM in every succession stage including some putative Methylocella/Methyloferula methanotrophs that are not detectable with the pmoA-based approach. In addition, a high diversity of 16S rRNA gene sequences likely representing cross-labeled nonmethanotrophs was discovered, including a significant proportion of Verrucomicrobia-related sequences. These results help to predict the effects of changing environmental conditions on SAM communities and activity.

Item Details

Item Type:Refereed Article
Keywords:methane oxidation, stable isotope probing, microarray, pmoA, 16S rRNA gene
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management not elsewhere classified
Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:117381
Year Published:2014
Web of Science® Times Cited:17
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-06-09
Last Modified:2017-09-04
Downloads:0

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