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Anion channel inhibitor NPPB-inhibited fluoride accumulation in tea plant (Camellia sinensis) is related to the regulation of Ca2+, CaM and depolarization of plasma membrane potential
Citation
Zhang, X-C and Gao, H-J and Yang, T-Y and Wu, H-H and Wang, Y-M and Zhang, Z-Z and Wan, X-C, Anion channel inhibitor NPPB-inhibited fluoride accumulation in tea plant (Camellia sinensis) is related to the regulation of Ca2+, CaM and depolarization of plasma membrane potential, International Journal of Molecular Sciences, 17, (1) Article 57. ISSN 1422-0067 (2016) [Refereed Article]
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Copyright Statement
Copyright 2016 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Abstract
Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca2+ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca2+ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca2+ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca2+ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca2+ and H+ efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca2+-CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Ca2+ efflux, Ca2+ fluorescence, CaM, fluoride, NPPB, tea plant |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Industrial crops |
| Objective Field: | Industrial crops not elsewhere classified |
| UTAS Author: | Wu, H-H (Mr Honghong Wu) |
| ID Code: | 117130 |
| Year Published: | 2016 |
| Web of Science® Times Cited: | 127 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2017-05-31 |
| Last Modified: | 2017-08-31 |
| Downloads: | 133 View Download Statistics |
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