eCite Digital Repository

Anion channel inhibitor NPPB-inhibited fluoride accumulation in tea plant (Camellia sinensis) is related to the regulation of Ca2+, CaM and depolarization of plasma membrane potential

Citation

Zhang, X-C and Gao, H-J and Yang, T-Y and Wu, H-H and Wang, Y-M and Zhang, Z-Z and Wan, X-C, Anion channel inhibitor NPPB-inhibited fluoride accumulation in tea plant (Camellia sinensis) is related to the regulation of Ca2+, CaM and depolarization of plasma membrane potential, International Journal of Molecular Sciences, 17, (1) Article 57. ISSN 1422-0067 (2016) [Refereed Article]


Preview
PDF
4Mb
  

Copyright Statement

Copyright 2016 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms17010057

Abstract

Tea plant is known to be a hyper-accumulator of fluoride (F). Over-intake of F has been shown to have adverse effects on human health, e.g., dental fluorosis. Thus, understanding the mechanisms fluoride accumulation and developing potential approaches to decrease F uptake in tea plants might be beneficial for human health. In the present study, we found that pretreatment with the anion channel inhibitor NPPB reduced F accumulation in tea plants. Simultaneously, we observed that NPPB triggered Ca2+ efflux from mature zone of tea root and significantly increased relative CaM in tea roots. Besides, pretreatment with the Ca2+ chelator (EGTA) and CaM antagonists (CPZ and TFP) suppressed NPPB-elevated cytosolic Ca2+ fluorescence intensity and CaM concentration in tea roots, respectively. Interestingly, NPPB-inhibited F accumulation was found to be significantly alleviated in tea plants pretreated with either Ca2+ chelator (EGTA) or CaM antagonists (CPZ and TFP). In addition, NPPB significantly depolarized membrane potential transiently and we argue that the net Ca2+ and H+ efflux across the plasma membrane contributed to the restoration of membrane potential. Overall, our results suggest that regulation of Ca2+-CaM and plasma membrane potential depolarization are involved in NPPB-inhibited F accumulation in tea plants.

Item Details

Item Type:Refereed Article
Keywords:Ca2+ efflux, Ca2+ fluorescence, CaM, fluoride, NPPB, tea plant
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Industrial Crops
Objective Field:Industrial Crops not elsewhere classified
Author:Wu, H-H (Mr Honghong Wu)
ID Code:117130
Year Published:2016
Web of Science® Times Cited:2
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-05-31
Last Modified:2017-08-31
Downloads:13 View Download Statistics

Repository Staff Only: item control page