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Acid-tolerant moderately thermophilic methanotrophs of the class gammaproteobacteria isolated from tropical topsoil with methane seeps
Citation
Islam, T and Torsvik, V and Larsen, O and Bodrossy, L and Ovreas, L and Birkeland, N-K, Acid-tolerant moderately thermophilic methanotrophs of the class gammaproteobacteria isolated from tropical topsoil with methane seeps, Frontiers in Microbiology, 7 Article 851. ISSN 1664-302X (2016) [Refereed Article]
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Copyright Statement
Copyright 2016 The Author(s) Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.3389/fmicb.2016.00851
Abstract
Terrestrial tropical methane seep habitats are important ecosystems in the methane cycle. Methane oxidizing bacteria play a key role in these ecosystems as they reduce methane emissions to the atmosphere. Here, we describe the isolation and initial characterization of two novel moderately thermophilic and acid-tolerant obligate methanotrophs, assigned BFH1 and BFH2 recovered from a tropical methane seep topsoil habitat. The new isolates were strictly aerobic, non-motile, coccus-shaped and utilized methane and methanol as sole carbon and energy source. Isolates grew at pH range 4.2–7.5 (optimal 5.5–6.0) and at a temperature range of 30–60°C (optimal 51–55°C). 16S rRNA gene phylogeny placed them in a well-separated branch forming a cluster together with the genus Methylocaldum as the closest relatives (93.1–94.1% sequence similarity). The genes pmoA, mxaF, and cbbL were detected, but mmoX was absent. Strains BFH1 and BFH2 are, to our knowledge, the first isolated acid-tolerant moderately thermophilic methane oxidizers of the class Gammaproteobacteria. Each strain probably denotes a novel species and they most likely represent a novel genus within the family Methylococcaceae of type I methanotrophs. Furthermore, the isolates increase our knowledge of acid-tolerant aerobic methanotrophs and signify a previously unrecognized biological methane sink in tropical ecosystems.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | terrestrial, Gammaproteobacteria, methanotroph, acid-tolerant, moderately thermophilic, pMMO |
| Research Division: | Biological Sciences |
| Research Group: | Microbiology |
| Research Field: | Microbial ecology |
| Objective Division: | Environmental Management |
| Objective Group: | Terrestrial systems and management |
| Objective Field: | Terrestrial systems and management not elsewhere classified |
| UTAS Author: | Bodrossy, L (Dr Levente Bodrossy) |
| ID Code: | 117107 |
| Year Published: | 2016 |
| Web of Science® Times Cited: | 15 |
| Deposited By: | Tasmanian Institute of Agriculture |
| Deposited On: | 2017-05-31 |
| Last Modified: | 2017-09-26 |
| Downloads: | 129 View Download Statistics |
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