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Acid-tolerant moderately thermophilic methanotrophs of the class gammaproteobacteria isolated from tropical topsoil with methane seeps


Islam, T and Torsvik, V and Larsen, O and Bodrossy, L and Ovreas, L and Birkeland, N-K, Acid-tolerant moderately thermophilic methanotrophs of the class gammaproteobacteria isolated from tropical topsoil with methane seeps, Frontiers in Microbiology, 7 Article 851. ISSN 1664-302X (2016) [Refereed Article]


Copyright Statement

Copyright 2016 The Author(s) Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.3389/fmicb.2016.00851


Terrestrial tropical methane seep habitats are important ecosystems in the methane cycle. Methane oxidizing bacteria play a key role in these ecosystems as they reduce methane emissions to the atmosphere. Here, we describe the isolation and initial characterization of two novel moderately thermophilic and acid-tolerant obligate methanotrophs, assigned BFH1 and BFH2 recovered from a tropical methane seep topsoil habitat. The new isolates were strictly aerobic, non-motile, coccus-shaped and utilized methane and methanol as sole carbon and energy source. Isolates grew at pH range 4.27.5 (optimal 5.56.0) and at a temperature range of 3060C (optimal 5155C). 16S rRNA gene phylogeny placed them in a well-separated branch forming a cluster together with the genus Methylocaldum as the closest relatives (93.194.1% sequence similarity). The genes pmoA, mxaF, and cbbL were detected, but mmoX was absent. Strains BFH1 and BFH2 are, to our knowledge, the first isolated acid-tolerant moderately thermophilic methane oxidizers of the class Gammaproteobacteria. Each strain probably denotes a novel species and they most likely represent a novel genus within the family Methylococcaceae of type I methanotrophs. Furthermore, the isolates increase our knowledge of acid-tolerant aerobic methanotrophs and signify a previously unrecognized biological methane sink in tropical ecosystems.

Item Details

Item Type:Refereed Article
Keywords:terrestrial, Gammaproteobacteria, methanotroph, acid-tolerant, moderately thermophilic, pMMO
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial ecology
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Terrestrial systems and management not elsewhere classified
UTAS Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:117107
Year Published:2016
Web of Science® Times Cited:15
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-05-31
Last Modified:2017-09-26
Downloads:129 View Download Statistics

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