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Field application of nitrogen and phenylacetylene to mitigate greenhouse gas emissions from landfill cover soils: effects on microbial community structure

Citation

Im, J and Lee, S-W and Bodrossy, L and Barcelona, MJ and Semrau, JD, Field application of nitrogen and phenylacetylene to mitigate greenhouse gas emissions from landfill cover soils: effects on microbial community structure, Applied Microbiology and Biotechnology, 89, (1) pp. 189-200. ISSN 0175-7598 (2011) [Refereed Article]

Copyright Statement

Copyright 2010 Springer-Verlag

DOI: doi:10.1007/s00253-010-2811-0

Abstract

Landfills are large sources of CH4, but a considerable amount of CH4 can be removed in situ by methanotrophs if their activity can be stimulated through the addition of nitrogen. Nitrogen can, however, lead to increased N2O production. To examine the effects of nitrogen and a selective inhibitor on CH4 oxidation and N2O production in situ, 0.5 M of NH4Cl and 0.25 M of KNO3, with and without 0.01% (w/v) phenylacetylene, were applied to test plots at a landfill in Kalamazoo, MI from 2007 November to 2009 July. Nitrogen amendments stimulated N2O production but had no effect on CH4 oxidation. The addition of phenylacetylene stimulated CH4 oxidation while reducing N2O production. Methanotrophs possessing particulate methane monooxygenase and archaeal ammonia-oxidizers (AOAs) were abundant. The addition of nitrogen reduced methanotrophic diversity, particularly for type I methanotrophs. The simultaneous addition of phenylacetylene increased methanotrophic diversity and the presence of type I methanotrophs. Clone libraries of the archaeal amoA gene showed that the addition of nitrogen increased AOAs affiliated with Crenarchaeal group 1.1b, while they decreased with the simultaneous addition of phenylacetylene. These results suggest that the addition of phenylacetylene with nitrogen reduces N2O production by selectively inhibiting AOAs and/or type II methanotrophs.

Item Details

Item Type:Refereed Article
Keywords:methane, nitrous oxide, methanotrophs, ammonia-oxidising archaea
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbial Ecology
Objective Division:Environment
Objective Group:Climate and Climate Change
Objective Field:Climate Change Mitigation Strategies
Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:117072
Year Published:2011
Web of Science® Times Cited:11
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-05-30
Last Modified:2017-08-18
Downloads:0

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