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Environmental distribution and abundance of the facultative methanotroph Methylocella
journal contribution
posted on 2023-05-19, 05:22 authored by Rahman, MT, Crombie, A, Chen, Y, Stralis-Pavese, N, Bodrossy, L, Meir, P, McNamara, NP, Murrell, JCMethylocella spp. are facultative methanotrophs, which are able to grow not only on methane but also on multicarbon substrates such as acetate, pyruvate or malate. Methylocella spp. were previously thought to be restricted to acidic soils such as peatlands, in which they may have a key role in methane oxidation. There is little information on the abundance and distribution of Methylocella spp. in the environment. New primers were designed, and a real-time quantitative PCR method was developed and validated targeting Methylocella mmoX (encoding the α-subunit of the soluble methane monooxygenase) that allowed the quantification of Methylocella spp. in environmental samples. We also developed and validated specific PCR assays, which target 16S rRNA genes of known Methylocella spp. These were used to investigate the distribution of Methylocella spp. in a variety of environmental samples. It was revealed that Methylocella species are widely distributed in nature and not restricted to acidic environments.
History
Publication title
ISME Journal: multidisciplinary journal of microbial ecologyVolume
5Pagination
1061-1066ISSN
1751-7362Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Nature Publishing GroupPlace of publication
4 Crinan St, London, N1 9XW United KingdomRights statement
Copyright 2011 International Society for Microbial Ecology All rights reservedRepository Status
- Restricted