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Methanotrophic bacteria in oilsands tailings ponds of northern Alberta

Citation

Saidi-Mehrabad, A and He, Z and Tamas, I and Sharp, CE and Brady, AL and Rochman, FF and Bodrossy, L and Abell, GCJ and Penner, T and Dong, X and Sensen, CW and Dunfield, PF, Methanotrophic bacteria in oilsands tailings ponds of northern Alberta, ISME Journal: multidisciplinary journal of microbial ecology, 7 pp. 908-921. ISSN 1751-7362 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 International Society for Microbial Ecology All rights reserved

DOI: doi:10.1038/ismej.2012.163

Abstract

We investigated methanotrophic bacteria in slightly alkaline surface water (pH 7.48.7) of oilsands tailings ponds in Fort McMurray, Canada. These large lakes (up to 10 km2) contain water, silt, clay and residual hydrocarbons that are not recovered in oilsands mining. They are primarily anoxic and produce methane but have an aerobic surface layer. Aerobic methane oxidation was measured in the surface water at rates up to 152 nmol CH4 ml−1 water d−1. Microbial diversity was investigated via pyrotag sequencing of amplified 16S rRNA genes, as well as by analysis of methanotroph-specific pmoA genes using both pyrosequencing and microarray analysis. The predominantly detected methanotroph in surface waters at all sampling times was an uncultured species related to the gammaproteobacterial genus Methylocaldum, although a few other methanotrophs were also detected, including Methylomonas spp. Active species were identified via 13CH4 stable isotope probing (SIP) of DNA, combined with pyrotag sequencing and shotgun metagenomic sequencing of heavy 13C-DNA. The SIP-PCR results demonstrated that the Methylocaldum and Methylomonas spp. actively consumed methane in fresh tailings pond water. Metagenomic analysis of DNA from the heavy SIP fraction verified the PCR-based results and identified additional pmoA genes not detected via PCR. The metagenome indicated that the overall methylotrophic community possessed known pathways for formaldehyde oxidation, carbon fixation and detoxification of nitrogenous compounds but appeared to possess only particulate methane monooxygenase not soluble methane monooxygenase.

Item Details

Item Type:Refereed Article
Keywords:oilsands, tailings pond, methane, methanotroph, Methylocaldum, microbial population, community ecology
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Community ecology (excl. invasive species ecology)
Objective Division:Environmental Management
Objective Group:Marine systems and management
Objective Field:Assessment and management of benthic marine ecosystems
UTAS Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:116638
Year Published:2013
Web of Science® Times Cited:59
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-05-15
Last Modified:2017-10-20
Downloads:0

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