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New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration
Citation
Duffy, E and Padovani, R and He, X and Gorkin, R and Vereshchagina, E and Ducree, J and Nesterenko, EP and Nesterenko, PN and Brabazon, D and Paull, B and Vazquez, M, New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration, Analytical Methods, 9, (13) pp. 1998-2006. ISSN 1759-9660 (2017) [Refereed Article]
Copyright Statement
Copyright 2017 The Royal Society of Chemistry
Abstract
New approaches for fabrication of centrifugal microfluidic platforms (μCDs) for sample micro-extraction and pre-concentration in bioanalytical and environmental applications are presented. The integration of both octadecylsilica (C18) micro-particulate and porous carbon monolithic stationary phases was demonstrated and on-disc extractions of analytes in samples of different nature were performed. A novel strategy based on the packing of micro-particulate stationary phases using porous organic polymer monoliths as column frits was demonstrated through the in situ photo-polymerisation of monolithic frits in a specific area of the micro-channel, thereby greatly facilitating stationary phase packing within μCD platforms. An enrichment factor of 3.7 was obtained for vitamin B12 following on-disc pre-concentration on the octadecylsilica columns. UV-Vis absorbance measurements were also performed in the outlet reservoir permitting quasi-on-line analysis of the small volume fractions collected after extraction, with limits of detection (LODs) found for vitamin B12 (LOD = 43 μM) being rather similar to those found with a commercially available spectrophotometer (LOD = 37 μM). Furthermore, the first integration of carbon monoliths within microfluidic channels is reported. Carbon monoliths were fabricated as rods and cut into discs for their integration within the microfluidic network, offering a highly porous bimodal structure with low flow-through back-pressures, excellent chemical stability as well as adequate mechanical stability. The carbon monolith-based μCD platform was evaluated as a rapid semi-automated pre-concentration approach suitable for in-field use prior to in-lab HPLC quantitation of pollutants at low concentration levels. Calculated mean recoveries for phenol from tap water spiked-samples by using this on-disc pre-concentration method were 68 ± 4% (n = 4, RSD = 5%).
Item Details
Item Type: | Refereed Article |
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Keywords: | centrifugal microfluidic platforms, stationary phase, sample preparation |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Nesterenko, PN (Professor Pavel Nesterenko) |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 116586 |
Year Published: | 2017 |
Web of Science® Times Cited: | 7 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2017-05-13 |
Last Modified: | 2018-03-27 |
Downloads: | 0 |
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