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New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration


Duffy, E and Padovani, R and He, X and Gorkin, R and Vereshchagina, E and Ducree, J and Nesterenko, EP and Nesterenko, PN and Brabazon, D and Paull, B and Vazquez, M, New strategies for stationary phase integration within centrifugal microfluidic platforms for applications in sample preparation and pre-concentration, Analytical Methods, 9, (13) pp. 1998-2006. ISSN 1759-9660 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 The Royal Society of Chemistry

DOI: doi:10.1039/c7ay00127d


New approaches for fabrication of centrifugal microfluidic platforms (μCDs) for sample micro-extraction and pre-concentration in bioanalytical and environmental applications are presented. The integration of both octadecylsilica (C18) micro-particulate and porous carbon monolithic stationary phases was demonstrated and on-disc extractions of analytes in samples of different nature were performed. A novel strategy based on the packing of micro-particulate stationary phases using porous organic polymer monoliths as column frits was demonstrated through the in situ photo-polymerisation of monolithic frits in a specific area of the micro-channel, thereby greatly facilitating stationary phase packing within μCD platforms. An enrichment factor of 3.7 was obtained for vitamin B12 following on-disc pre-concentration on the octadecylsilica columns. UV-Vis absorbance measurements were also performed in the outlet reservoir permitting quasi-on-line analysis of the small volume fractions collected after extraction, with limits of detection (LODs) found for vitamin B12 (LOD = 43 μM) being rather similar to those found with a commercially available spectrophotometer (LOD = 37 μM). Furthermore, the first integration of carbon monoliths within microfluidic channels is reported. Carbon monoliths were fabricated as rods and cut into discs for their integration within the microfluidic network, offering a highly porous bimodal structure with low flow-through back-pressures, excellent chemical stability as well as adequate mechanical stability. The carbon monolith-based μCD platform was evaluated as a rapid semi-automated pre-concentration approach suitable for in-field use prior to in-lab HPLC quantitation of pollutants at low concentration levels. Calculated mean recoveries for phenol from tap water spiked-samples by using this on-disc pre-concentration method were 68 4% (n = 4, RSD = 5%).

Item Details

Item Type:Refereed Article
Keywords:centrifugal microfluidic platforms, stationary phase, sample preparation
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Nesterenko, PN (Professor Pavel Nesterenko)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:116586
Year Published:2017
Web of Science® Times Cited:9
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2017-05-13
Last Modified:2018-03-27

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