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Water dispersal of methanotrophic bacteria maintains functional methane oxidation in Sphagnum mosses

Citation

Putkinen, A and Larmola, T and Tuomivirta, T and Siljanen, HMP and Bodrossy, L and Tuittila, ES and Fritze, H, Water dispersal of methanotrophic bacteria maintains functional methane oxidation in Sphagnum mosses, Frontiers in Microbiology, 3 Article 15. ISSN 1664-302X (2012) [Refereed Article]


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Copyright 2012 The Author(s) Licensed under Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) https://creativecommons.org/licenses/by-nc/3.0/

DOI: doi:10.3389/fmicb.2012.00015

Abstract

It is known that Sphagnum associated methanotrophy (SAM) changes in relation to the peatland water table (WT) level. After drought, rising WT is able to reactivate SAM. We aimed to reveal whether this reactivation is due to activation of indigenous methane (CH4) oxidizing bacteria (MOB) already present in the mosses or to MOB present in water. This was tested through two approaches: in a transplantation experiment, Sphagna lacking SAM activity were transplanted into flark water next to Sphagna oxidizing CH4. Already after 3 days, most of the transplants showed CH4 oxidation activity. Microarray showed that the MOB community compositions of the transplants and the original active mosses had become more similar within 28 days thus indicating MOB movement through water between mosses. Methylocystis-related type II MOB dominated the community. In a following experiment, SAM inactive mosses were bathed overnight in non-sterile and sterile-filtered SAM active site flark water. Only mosses bathed with non-sterile flark water became SAM active, which was also shown by the pmoA copy number increase of over 60 times. Thus, it was evident that MOB present in the water can colonize Sphagnum mosses. This colonization could act as a resilience mechanism for peatland CH4 dynamics by allowing the re-emergence of CH4 oxidation activity in Sphagnum.

Item Details

Item Type:Refereed Article
Keywords:plant-microbe interaction, peatland, pmoA, microarray, qPCR, ecosystem resilience
Research Division:Environmental Sciences
Research Group:Ecological Applications
Research Field:Ecosystem Function
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Fresh, Ground and Surface Water Environments
Author:Bodrossy, L (Dr Levente Bodrossy)
ID Code:116515
Year Published:2012
Web of Science® Times Cited:15
Deposited By:Plant Science
Deposited On:2017-05-11
Last Modified:2017-09-26
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