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Optical coherence tomographybased contactindentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease

Citation

Wang, KC and Astell, CJ and Wijesinghe, P and Larcombe, AN and Pinniger, GJ and Zosky, GR and Kennedy, BF and Berry, LJ and Sampson, DD and James, AL and Le Cras, TD and Noble, PB, Optical coherence tomographybased contactindentation for diaphragm mechanics in a mouse model of transforming growth factor alpha induced lung disease, Scientific reports, 7 pp. 1-10. ISSN 2045-2322 (2017) [Refereed Article]


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Copyright 2017 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/s41598-017-01431-x

Abstract

This study tested the utility of optical coherence tomography (OCT)-based indentation to assess mechanical properties of respiratory tissues in disease. Using OCT-based indentation, the elastic modulus of mouse diaphragm was measured from changes in diaphragm thickness in response to an applied force provided by an indenter. We used a transgenic mouse model of chronic lung disease induced by the overexpression of transforming growth factor-alpha (TGF-α), established by the presence of pleural and peribronchial fibrosis and impaired lung mechanics determined by the forced oscillation technique and plethysmography. Diaphragm elastic modulus assessed by OCT-based indentation was reduced by TGF-α at both left and right lateral locations (p<0.05). Diaphragm elastic modulus at left and right lateral locations were correlated within mice (r=0.67, p<0.01) suggesting that measurements were representative of tissue beyond the indenter field. Co-localised images of diaphragm after TGF-α overexpression revealed a layered fibrotic appearance. Maximum diaphragm force in conventional organ bath studies was also reduced by TGF-α overexpression (p<0.01). Results show that OCT-based indentation provided clear delineation of diseased diaphragm, and together with organ bath assessment, provides new evidence suggesting that TGF-α overexpression produces impairment in diaphragm function and, therefore, an increase in the work of breathing in chronic lung disease.

Item Details

Item Type:Refereed Article
Keywords:TGF-a, optical coherence tomography, diaphragm function
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Respiratory Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Respiratory System and Diseases (incl. Asthma)
Author:Zosky, GR (Associate Professor Graeme Zosky)
ID Code:116338
Year Published:2017
Deposited By:Medicine (Discipline)
Deposited On:2017-05-05
Last Modified:2017-08-22
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