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Fractal kinetic behavior of plasmin on the surface of fibrin meshwork
Citation
Varju, I and Tenekedjiev, K and Keresztes, Z and Pap, AE and Szabo, L and Thelwell, C and Longstaff, C and Machovich, R and Kolev, K, Fractal kinetic behavior of plasmin on the surface of fibrin meshwork, Biochemistry, 53, (40) pp. 6348-6356. ISSN 0006-2960 (2014) [Refereed Article]
Copyright Statement
© 2014 American Chemical Society
Abstract
Intravascular fibrin clots are resolved by plasmin acting at the interface of gel-phase substrate and fluid-borne enzyme. The classic Michaelis–Menten kinetic scheme cannot describe satisfactorily this heterogeneous-phase proteolysis because it assumes homogeneous well-mixed conditions. A more suitable model for these spatial constraints, known as fractal kinetics, includes a time-dependence of the Michaelis coefficient KmF = Km0F(1 + t)h, where h is a fractal exponent of time, t. The aim of the present study was to build up and experimentally validate a mathematical model for surface-acting plasmin that can contribute to a better understanding of the factors that influence fibrinolytic rates. The kinetic model was fitted to turbidimetric data for fibrinolysis under various conditions. The model predicted Km0F= 1.98 μM and h = 0.25 for fibrin composed of thin fibers and Km0F= 5.01 μM and h = 0.16 for thick fibers in line with a slower macroscale lytic rate (due to a stronger clustering trend reflected in the h value) despite faster cleavage of individual thin fibers (seen as lower Km0F). ε-Aminocaproic acid at 1 mM or 8 U/mL carboxypeptidase-B eliminated the time-dependence of KmF and increased the lysis rate suggesting a role of C-terminal lysines in the progressive clustering of plasmin. This fractal kinetic concept gained structural support from imaging techniques. Atomic force microscopy revealed significant changes in plasmin distribution on a patterned fibrinogen surface in line with the time-dependent clustering of fluorescent plasminogen in confocal laser microscopy. These data from complementary approaches support a mechanism for loss of plasmin activity resulting from C-terminal lysine-dependent redistribution of enzyme molecules on the fibrin surface.
Item Details
Item Type: | Refereed Article |
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Keywords: | mathematical modelling, simulation, fractal kinetic, fibrin meshwork |
Research Division: | Mathematical Sciences |
Research Group: | Statistics |
Research Field: | Applied statistics |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the information and computing sciences |
UTAS Author: | Tenekedjiev, K (Professor Kiril Tenekedjiev) |
ID Code: | 116180 |
Year Published: | 2014 |
Web of Science® Times Cited: | 12 |
Deposited By: | NC Maritime Engineering and Hydrodynamics |
Deposited On: | 2017-05-03 |
Last Modified: | 2017-11-01 |
Downloads: | 0 |
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