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Fractal kinetic behavior of plasmin on the surface of fibrin meshwork

Citation

Varju, I and Tenekedjiev, K and Keresztes, Z and Pap, AE and Szabo, L and Thelwell, C and Longstaff, C and Machovich, R and Kolev, K, Fractal kinetic behavior of plasmin on the surface of fibrin meshwork, Biochemistry, 53, (40) pp. 6348-6356. ISSN 0006-2960 (2014) [Refereed Article]

Copyright Statement

© 2014 American Chemical Society

DOI: doi:10.1021/bi500661m

Abstract

Intravascular fibrin clots are resolved by plasmin acting at the interface of gel-phase substrate and fluid-borne enzyme. The classic Michaelis–Menten kinetic scheme cannot describe satisfactorily this heterogeneous-phase proteolysis because it assumes homogeneous well-mixed conditions. A more suitable model for these spatial constraints, known as fractal kinetics, includes a time-dependence of the Michaelis coefficient KmF = Km0F(1 + t)h, where h is a fractal exponent of time, t. The aim of the present study was to build up and experimentally validate a mathematical model for surface-acting plasmin that can contribute to a better understanding of the factors that influence fibrinolytic rates. The kinetic model was fitted to turbidimetric data for fibrinolysis under various conditions. The model predicted Km0F= 1.98 μM and h = 0.25 for fibrin composed of thin fibers and Km0F= 5.01 μM and h = 0.16 for thick fibers in line with a slower macroscale lytic rate (due to a stronger clustering trend reflected in the h value) despite faster cleavage of individual thin fibers (seen as lower Km0F). ε-Aminocaproic acid at 1 mM or 8 U/mL carboxypeptidase-B eliminated the time-dependence of KmF and increased the lysis rate suggesting a role of C-terminal lysines in the progressive clustering of plasmin. This fractal kinetic concept gained structural support from imaging techniques. Atomic force microscopy revealed significant changes in plasmin distribution on a patterned fibrinogen surface in line with the time-dependent clustering of fluorescent plasminogen in confocal laser microscopy. These data from complementary approaches support a mechanism for loss of plasmin activity resulting from C-terminal lysine-dependent redistribution of enzyme molecules on the fibrin surface.

Item Details

Item Type:Refereed Article
Keywords:mathematical modelling, simulation, fractal kinetic, fibrin meshwork
Research Division:Mathematical Sciences
Research Group:Statistics
Research Field:Applied Statistics
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Information and Computing Sciences
Author:Tenekedjiev, K (Professor Kiril Tenekedjiev)
ID Code:116180
Year Published:2014
Web of Science® Times Cited:5
Deposited By:NC Maritime Engineering and Hydrodynamics
Deposited On:2017-05-03
Last Modified:2017-11-01
Downloads:0

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