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116003 - Early CCR6 expression on B cells - Final author version.pdf (182.56 kB)

Early CCR6 expression on B cells modulates germinal centre kinetics and efficient antibody responses

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posted on 2023-05-19, 04:00 authored by Reimer, D, Adrian Lee, Bannan, J, Fromm, P, Kara, EE, Comerford, I, McColl, S, Wiede, F, Mielenz, D, Heinrich KornerHeinrich Korner
The CC-chemokine receptor 6 (CCR6) can be detected on naive and activated B cells. Counterintuitively, its absence accelerates the appearance of germinal centres (GCs) and increases the production of low-affinity antibodies. The detailed mechanism of CCR6 function during the humoral response has remained elusive, but previously we identified a distinct CCR6high B-cell population in vivo early after antigenic challenge. In this study, we defined this population specifically as early, activated pre-GC B cells. In accordance, we show that CCR6 is upregulated rapidly within hours on the protein or mRNA level after activation in vitro. In addition, only activated B cells migrated specifically towards CCL20, the specific ligand for CCR6. Lack of CCR6 increased the dark zone/light zone ratio of GC and led to decreased antigen-specific IgG1 and IgG2a antibody generation in a B-cell intrinsic manner in mixed bone marrow chimeras. In contrast, antigen-specific IgM responses were normal. Hence, CCR6 negatively regulates entry of activated, antigen-specific pre-GC B cells into the GC reaction.

History

Publication title

Immunology and Cell Biology

Volume

95

Pagination

33-41

ISSN

0818-9641

Department/School

Menzies Institute for Medical Research

Publisher

Nature Publishing Group

Place of publication

United Kingdom

Rights statement

Copyright 2016 Australasian Society for Immunology Inc.

Repository Status

  • Open

Socio-economic Objectives

Clinical health not elsewhere classified

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