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Assessing sequence diversity of groundnut rosette disease agents and the distribution of Groundnut rosette assistor virus in major groundnut-producing regions of Ghana

Citation

Appiah, AS and Sossah, FL and Tegg, RS and Offei, SK and Wilson, CR, Assessing sequence diversity of groundnut rosette disease agents and the distribution of Groundnut rosette assistor virus in major groundnut-producing regions of Ghana, Tropical plant pathology, 42, (2) pp. 109-120. ISSN 1982-5676 (2017) [Refereed Article]


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Copyright 2017 Sociedade Brasileira de Fitopatologia

DOI: doi:10.1007/s40858-017-0140-x

Abstract

The incidence of Groundnut rosette assistor virus (GRAV) in farmers’ fields and sequence diversity of groundnut rosette disease (GRD) agents were assessed in the three northern groundnut production regions of Ghana. GRAV incidence was high (69.5 to 75.0%) but not significantly different between the regions. Nucleotide sequencing of GRAV coat protein (CP) gene revealed 99–100% identity among the Ghanaian isolates and 97–100% similarity to GRAV sequences from Nigeria and Malawi for both nucleotide and predicted amino acids. Nucleotide sequence identities of partial ORF3 and 4 of Groundnut rosette virus (GRV) among the Ghanaian isolates were more variable (89–100%). Ghanaian GRV isolates were more closely related in nucleotide sequence identity to Nigerian isolates (95–98%) than Malawian isolates (88–90%). Similarly, nucleotide identity within Ghanaian GRV-sat RNA’s were close (94–100%), but distinct from Nigerian (82–87%) and Malawian (82–86%) GRV-sat RNAs. Ghanaian isolates of all three agents of GRD showed no obvious isolate diversity patterns based on the regions from where they were collected. We present the first report on the distribution and genetic diversity of GRD agents in Ghana.

Item Details

Item Type:Refereed Article
Keywords:Arachis hypogaea, groundnut rosette assistor virus, groundnut rosette virus, field survey, groundnut rosette disease, sequence analysis
Research Division:Agricultural and Veterinary Sciences
Research Group:Horticultural Production
Research Field:Horticultural Crop Protection (Pests, Diseases and Weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Horticultural Crops not elsewhere classified
Author:Appiah, AS (Mr Andrew Appiah)
Author:Tegg, RS (Dr Robert Tegg)
Author:Wilson, CR (Associate Professor Calum Wilson)
ID Code:115925
Year Published:2017
Deposited By:TIA - Research Institute
Deposited On:2017-04-21
Last Modified:2017-05-15
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