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Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide


Chen, L and Eapen, MS and Zosky, GR, Vitamin D both facilitates and attenuates the cellular response to lipopolysaccharide, Scientific Reports, 7 Article 45172. ISSN 2045-2322 (2017) [Refereed Article]


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Copyright 2017 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1038/srep45172


Vitamin D has a range of non-skeletal health effects and has been implicated in the response to respiratory infections. The aim of this study was to assess the effect of vitamin D on the response of epithelial cells, neutrophils and macrophages to lipopolysaccharide (LPS) stimulation. BEAS-2B cells (airway epithelial cell line) and primary neutrophils and macrophages isolated from blood samples were cultured and exposed to LPS with and without vitamin D (1,25(OH)2D). The production of IL-6, IL-8, IL-1β and TNF-α of all cells and the phagocytic capacity of neutrophils and macrophages to E. coli were assessed. Vitamin D had no effect on BEAS-2B cells but enhanced the production of IL-8 in neutrophils (p = 0.007) and IL-1β in macrophages (p = 0.007) in response to LPS. Both vitamin D (p = 0.019) and LPS (p < 0.001) reduced the phagocytic capacity of macrophages. These data suggest that the impact of vitamin D on responses to infection are complex and that the net effect will depend on the cells that respond, the key response that is necessary for resolution of infection (cytokine production or phagocytosis) and whether there is pre-existing inflammation.

Item Details

Item Type:Refereed Article
Keywords:vitamin D, LPS, cytokines, neutrophils, macrophages, epithelial cells
Research Division:Biomedical and Clinical Sciences
Research Group:Cardiovascular medicine and haematology
Research Field:Respiratory diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Chen, L (Dr Ling Chen)
UTAS Author:Eapen, MS (Dr Mathew Eapen)
UTAS Author:Zosky, GR (Professor Graeme Zosky)
ID Code:115627
Year Published:2017
Funding Support:National Health and Medical Research Council (1042235)
Web of Science® Times Cited:25
Deposited By:Medicine
Deposited On:2017-04-03
Last Modified:2022-08-25
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