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3D printed LED based on-capillary detector housing with integrated slit

Citation

Cecil, F and Zhang, M and Guijt, RM and Henderson, A and Nesterenko, PN and Paull, B and Breadmore, MC and Macka, M, 3D printed LED based on-capillary detector housing with integrated slit, Analytica Chimica Acta, 965 pp. 131-136. ISSN 0003-2670 (2017) [Refereed Article]

Copyright Statement

2017 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.aca.2017.02.020

Abstract

A 3D printed photometric detector body with integrated slit was fabricated to position a LED and photodiode either side of capillary tubing using a fused deposition modelling (FDM) printer. To make this approach suitable for capillaries down to 50μm i.d. the dimension of the in-built slit is the critical element of the printed housing. The spatial orientation of the model for printing was found to significantly impact on the resolution of the structures and voids that can be printed. By designing a housing with a slit positioned in the XY plane in parallel with the print direction, the narrowest void (slit) that could be printed was 70μm. The potential use of the 3D printed slit for photometric detection was characterised using tubing and capillary from 500 down to 50μm i.d, demonstrating a linear response from 632 to 40 mAU. The effective pathlength and stray light varied from 383 to 22μm and 3.8% - 50% for 500- 50μm i.d tubing and capillary. The use of a V-shaped alignment feature allowed for easy and reliable positioning of the tubing inside the detector, as demonstrated by a RSD of 1.9% (n=10) in peak height when repositioning the tubing between measurements using flow injection analysis (FIA). The performance of the 3D printed housing and 70μm slit was benchmarked against a commercially available interface using the CE separation of Zn2+ and Cu2+ complexes with PAR. The limit of detection with the 3D printed slit was 6.8 and 4.5μM and is 2.8 and 1.6μM with the commercial interface.

Item Details

Item Type:Refereed Article
Keywords:3D printing, fused deposition modelling, light emitting diode, LED on-capillary detector, flow injection analysis, capillary electrophoresis
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Instrumental Methods (excl. Immunological and Bioassay Methods)
Objective Division:Environment
Objective Group:Physical and Chemical Conditions of Water
Objective Field:Physical and Chemical Conditions of Water in Marine Environments
Author:Cecil, F (Mr Farhan Cecil)
Author:Zhang, M (Dr Min Zhang)
Author:Guijt, RM (Dr Rosanne Guijt)
Author:Henderson, A (Dr Alan Henderson)
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
Author:Breadmore, MC (Professor Michael Breadmore)
Author:Macka, M (Professor Mirek Macka)
ID Code:115412
Year Published:2017
Deposited By:Chemistry
Deposited On:2017-03-23
Last Modified:2017-11-15
Downloads:0

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