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The growing and glowing toolbox of fluorescent and photoactive proteins
Citation
Rodriguez, EA and Campbell, RE and Lin, JY and Lin, MZ and Miyawaki, A and Palmer, AE and Shu, X and Zhang, J and Tsien, RY, The growing and glowing toolbox of fluorescent and photoactive proteins, Trends in Biochemical Sciences, 42, (2) pp. 111-129. ISSN 0968-0004 (2017) [Refereed Article]
Copyright Statement
Copyright 2016 Elsevier Ltd.
DOI: doi:10.1016/j.tibs.2016.09.010
Abstract
Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | fluorescent protein, microscopy, protein engineering and design |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Medical biotechnology |
| Research Field: | Medical molecular engineering of nucleic acids and proteins |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the biological sciences |
| UTAS Author: | Lin, JY (Dr John Lin) |
| ID Code: | 115392 |
| Year Published: | 2017 |
| Web of Science® Times Cited: | 279 |
| Deposited By: | Medicine |
| Deposited On: | 2017-03-22 |
| Last Modified: | 2019-09-23 |
| Downloads: | 0 |
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