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The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Citation

Rodriguez, EA and Campbell, RE and Lin, JY and Lin, MZ and Miyawaki, A and Palmer, AE and Shu, X and Zhang, J and Tsien, RY, The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins, Trends in biochemical sciences, 42, (2) pp. 111-129. ISSN 0968-0004 (2017) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier Ltd.

DOI: doi:10.1016/j.tibs.2016.09.010

Abstract

Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.

Item Details

Item Type:Refereed Article
Keywords:fluorescent protein, microscopy, protein engineering and design
Research Division:Technology
Research Group:Medical Biotechnology
Research Field:Medical Molecular Engineering of Nucleic Acids and Proteins
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Lin, JY (Dr John Lin)
ID Code:115392
Year Published:2017
Web of Science® Times Cited:18
Deposited By:Medicine (Discipline)
Deposited On:2017-03-22
Last Modified:2017-05-15
Downloads:0

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