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Definition of the minimal MEN1 candidate area based on a 5-Mb integrated map of proximal 11q13 - The European consortium on MEN1

Citation

Courseaux, A and Grosgeorge, J and Gaudray, P and Pannett, AAJ and Forbes, SA and Williamson, C and Bassett, D and Thakker, RV and Teh, BT and Farnebo, F and Shepherd, JJ and Skogseid, B and Larsson, C and Giraud, S and Zhang, CX and Salandre, J and Calender, A, Definition of the minimal MEN1 candidate area based on a 5-Mb integrated map of proximal 11q13 - The European consortium on MEN1, Genomics, 37, (3) pp. 354-365. ISSN 0888-7543 (1996) [Refereed Article]

DOI: doi:10.1006/geno.1996.0570

Abstract

Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant disorder with a high penetrance characterized by tumors of the parathyroid glands, the endocrine pancreas, and the anterior pituitary. The MEN1 gene, a putative tumor suppressor gene, has been mapped to a 3- to 8-cM region in chromosome 11q13 but it remains elusive as yet. We have combined the efforts and resources from four laboratories to form the European Consortium on MEN1 with the aims of establishing the genetic and the physical maps of 11q13 and of further narrowing the MEN1 region. A 5-Mb integrated map of the region was established by fluorescence in situ hybridization on both metaphase chromosomes and DNA fibers, by hybridization to DNA from somatic cell hybrids containing various parts of human chromosome 11, by long-range restriction mapping, and by characterization of YACs and cosmids. Polymorphic markers were positioned and ordered by physical mapping and genetic linkage in 86 MEN1 families with 452 affected individuals. Two critical recombinants identified in two affected cases placed the MEN1 gene in an ≃2-Mb region around PYGM, flanked by D11S1883 and D11S449.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Medical Genetics (excl. Cancer Genetics)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Inherited Diseases (incl. Gene Therapy)
Author:Shepherd, JJ (Professor Joseph Shepherd)
ID Code:11537
Year Published:1996
Web of Science® Times Cited:83
Deposited By:Surgery
Deposited On:1997-08-01
Last Modified:2011-08-22
Downloads:0

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