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Precocious floral initiation and identification of exact timing of embryo physiological maturity facilitate germination of immature seeds to truncate the lifecycle of pea

Citation

Ribalta, FM and Pazos-Navarro, MP and Nelson, K and Edwards, K and Ross, JJ and Bennett, RG and Munday, C and Erskine, W and Ochatt, SJ and Croser, JS, Precocious floral initiation and identification of exact timing of embryo physiological maturity facilitate germination of immature seeds to truncate the lifecycle of pea, Plant Growth Regulation, 81, (2) pp. 345-353. ISSN 0167-6903 (2017) [Refereed Article]

Copyright Statement

Copyright 2016 Springer Science+Business Media Dordrech

DOI: doi:10.1007/s10725-016-0211-x

Abstract

We propose herein a novel single seed descent protocol that has application across a broad phenotypic range of pea genotypes. Manipulation of key in vivo growing conditions, including light, photoperiod and temperature, combined with precocious in vitro germination of the embryo at full physiological maturity substantially shortened the pea lifecycle. We define full embryo physiological maturity as the earliest point in seed development when precocious in vitro germination and robust seedling growth can be reliably achieved without supply of exogenous hormones. Under our optimised conditions for accelerated plant growth, embryo physiological maturity was attained at c. 18 days after pollination, when seed moisture content was below 60 % and sucrose level under 100 mg g−1 DW. No delay penalty in terms of time to flowering and plant development was caused by the culture of immature seeds 18 days after pollination compared to the used of mature ones. Determining the role embryo maturity plays in the fitness of the germinated plant has facilitated the truncation of the lifecycle across pea genotypes. The accelerated single seed descent system proposed within this research will benefit complex genetic studies via the rapid development of recombinant inbred lines (RIL) and multi-parental advanced generation intercrosses (MAGIC) populations.

Item Details

Item Type:Refereed Article
Keywords:Pisum sativum, germination, seeds.
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Ross, JJ (Associate Professor John Ross)
ID Code:115195
Year Published:2017 (online first 2016)
Funding Support:Australian Research Council (DP130103357)
Web of Science® Times Cited:4
Deposited By:Plant Science
Deposited On:2017-03-09
Last Modified:2017-11-02
Downloads:0

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