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Nanoporous membranes for microfluidic concentration prior to electrophoretic separation of proteins in urine

Citation

Li, F and Guijt, RM and Breadmore, MC, Nanoporous membranes for microfluidic concentration prior to electrophoretic separation of proteins in urine, Analytical Chemistry, 88, (16) pp. 8257-8263. ISSN 0003-2700 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 American Chemical Society

DOI: doi:10.1021/acs.analchem.6b02096

Abstract

A microfluidic device with two nanoporous membranes was developed to seamlessly integrate sample preparation and electrophoretic separation of proteins. The device was fabricated by sandwiching two nanoporous polycarbonate track etched (PCTE) membranes with differently sized nanopores between PDMS slabs containing embedded microchannels. The first membrane contained larger (100 nm) pores and served as an initial filter to screen out particles, cells and larger proteins. The second membrane contained smaller pores (10 nm) which facilitated transport of inorganic ions and small organic molecules, but not proteins. The sequential combination of these two membranes allows proteins to be concentrated and purified simultaneously. The device was used for the sample-in/answer-out quantification of albumin in human urine within 2.5 min with an improvement in sensitivity of 500 fold compared to a normal pinched injection using fluorescence detection. The linear range of was 0100 μg mL1, with a LOD of 1.5 μg mL1 covering the diagnostic level of microalbuminuria of 30 μg mL1. The presented device, which is simple to make and use, provides a quantitative alternative for point-of-care detection of proteins, as demonstrated through its application to albumin in urine for the diagnoisis of (micro)albuminuria.

Item Details

Item Type:Refereed Article
Keywords:microchip
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Li, F (Mr Feng Li)
Author:Guijt, RM (Dr Rosanne Guijt)
Author:Breadmore, MC (Professor Michael Breadmore)
ID Code:115082
Year Published:2016
Funding Support:Australian Research Council (FT130100101)
Web of Science® Times Cited:5
Deposited By:Chemistry
Deposited On:2017-03-07
Last Modified:2017-10-30
Downloads:0

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