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Elution, detection, and quantification of polio I, bacteriophages, Salmonella Montevideo, and Escherichia coli O157:H7 from seeded strawberries and tomatoes

Citation

Lukasik, J and Bradley, ML and Scott, TM and Hsu, W-Y and Farrah, SR and Tamplin, ML, Elution, detection, and quantification of polio I, bacteriophages, Salmonella Montevideo, and Escherichia coli O157:H7 from seeded strawberries and tomatoes, Journal of Food Protection, 64, (3) pp. 292-297. ISSN 0362-028X (2001) [Refereed Article]

Copyright Statement

Copyright 2001 International Association for Food Protection

DOI: doi:10.4315/0362-028X-64.3.292

Abstract

This study compared the effect of different physical and chemical treatments of strawberries and tomatoes to determine their ability to recover seeded viral and bacterial pathogens from produce surfaces. Solutions of salts, amino acids, complex media, and detergents were compared as eluants. Phosphate-buffered saline (PBS) containing 0.1% Tween 80 eluted the highest number of seeded microorganisms. Elution with this defined solution was then compared under different conditions of physical agitation. Rotary shaking for 20 min at 36C eluted higher numbers of viruses and bacteria than did low- or high-speed stomaching. Commercially available and laboratory prepared bacteriological differential media were compared for their ability to recover and distinguish eluted Salmonella Montevideo and Escherichia coli O157:H7 strains from seeded produce. The recovery of seeded bacterial pathogens was low when differential media containing selective ingredients were used (Mac-Conkey sorbitol agar, XLD agar, MacConkey agar). Highest recoveries were obtained on a medium consisting of tryptic soy agar supplemented with sodium thiosulfate and ferric ammonium citrate compared with selective media that inhibited up to 50% of the growth of the eluted microorganisms.

Item Details

Item Type:Refereed Article
Research Division:Engineering
Research Group:Food Sciences
Research Field:Food Packaging, Preservation and Safety
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Tamplin, ML (Professor Mark Tamplin)
ID Code:115074
Year Published:2001
Web of Science® Times Cited:29
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2017-03-07
Last Modified:2017-05-01
Downloads:0

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