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Online extraction coupled to liquid chromatography analysis (OLELC): eliminating traditional sample preparation steps in the investigation of solid complex matrices
Citation
Ferreira, VG and Leme, GM and Cavalheiro, AJ and Funari, CS, Online extraction coupled to liquid chromatography analysis (OLELC): eliminating traditional sample preparation steps in the investigation of solid complex matrices, Analytical Chemistry, 88, (17) pp. 8421-8427. ISSN 0003-2700 (2016) [Refereed Article]
Copyright Statement
Copyright 2016 American Chemical Society
DOI: doi:10.1021/acs.analchem.6b02388
Abstract
Current methods employed for the analysis of the chemical composition of solid matrices (such as plant, animal, or human tissues; soil; etc.) often require many sample treatment steps, including an extraction step with exclusively dedicated solvents. This work describes an optimized analytical setup in which the extraction of a solid sample is directly coupled to its analysis by high-performance liquid chromatography. This approach avoids (i) the use of pumps and valves other than those comprising the HPLC instrument, (ii) the use of solvents other than those of the mobile phase, and (iii) the need to stop the mobile phase flow at any time during the full analytical procedure. The compatibility of this approach with the direct analysis of fresh tissues (leaves, stems, and seeds of four plant species with dissimilar chemical compositions) was successfully demonstrated, leading to the elimination of sample preparation steps such as drying, grinding, concentration, dilution, and filtration, among others. This work describes a new, simple, and efficient green approach to minimize or eliminate sample treatment procedures. It could be easily applied for quality control of plant materials and their derived products through chromatographic fingerprints and for untargeted metabolomic investigations of solid matrices, among other applications.
Item Details
Item Type: | Refereed Article |
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Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Instrumental methods (excl. immunological and bioassay methods) |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Funari, CS (Dr Cristiano Funari) |
ID Code: | 115041 |
Year Published: | 2016 |
Web of Science® Times Cited: | 16 |
Deposited By: | Chemistry |
Deposited On: | 2017-03-06 |
Last Modified: | 2017-09-04 |
Downloads: | 0 |
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