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Online extraction coupled to liquid chromatography analysis (OLELC): eliminating traditional sample preparation steps in the investigation of solid complex matrices

Citation

Ferreira, VG and Leme, GM and Cavalheiro, AJ and Funari, CS, Online extraction coupled to liquid chromatography analysis (OLELC): eliminating traditional sample preparation steps in the investigation of solid complex matrices, Analytical Chemistry, 88, (17) pp. 8421-8427. ISSN 0003-2700 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 American Chemical Society

DOI: doi:10.1021/acs.analchem.6b02388

Abstract

Current methods employed for the analysis of the chemical composition of solid matrices (such as plant, animal, or human tissues; soil; etc.) often require many sample treatment steps, including an extraction step with exclusively dedicated solvents. This work describes an optimized analytical setup in which the extraction of a solid sample is directly coupled to its analysis by high-performance liquid chromatography. This approach avoids (i) the use of pumps and valves other than those comprising the HPLC instrument, (ii) the use of solvents other than those of the mobile phase, and (iii) the need to stop the mobile phase flow at any time during the full analytical procedure. The compatibility of this approach with the direct analysis of fresh tissues (leaves, stems, and seeds of four plant species with dissimilar chemical compositions) was successfully demonstrated, leading to the elimination of sample preparation steps such as drying, grinding, concentration, dilution, and filtration, among others. This work describes a new, simple, and efficient green approach to minimize or eliminate sample treatment procedures. It could be easily applied for quality control of plant materials and their derived products through chromatographic fingerprints and for untargeted metabolomic investigations of solid matrices, among other applications.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Instrumental Methods (excl. Immunological and Bioassay Methods)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Funari, CS (Dr Cristiano Funari)
ID Code:115041
Year Published:2016
Deposited By:Chemistry
Deposited On:2017-03-06
Last Modified:2017-09-04
Downloads:0

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