A coacervative extraction (CAE) with the common cationic surfactants dodecyltrimethylammonium bromide (DTAB) and didodecyldimethylammonium bromide (DDAB) was firstly developed. Notable characteristics of the CAE was the use of low concentration of salt (0.1 mol L⁻¹ NaBr) at ambient temperature and without the requirement of organic solvent. The CAE was based on phase separation due to the neutralization of the surface charge of the micelle by electrostatic interaction with the predominant common counter ion (bromide). The coacervative phase was subjected to an optimized micellar liquid chromatography with UV (MLC-UV) method without any treatments. The method was applied to the determination of penicillins including amoxicillin, ampicillin, penicillin-G, oxacillin, and cloxacillin in milk samples. Method detection limits (MDLs) from standard were 0.5–2 ng mL⁻¹, and 40–80-fold analyte enrichment were obtained. The CAE-MLC-UV has shown to be of high potential for the analysis of five penicillins in milk with recoveries >90%.

Item Type:	Refereed Article
Keywords:	analysis
Research Division:	Chemical Sciences
Research Group:	Analytical Chemistry
Research Field:	Separation Science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding Knowledge
Objective Field:	Expanding Knowledge in the Chemical Sciences
UTAS Author:	Quirino, JP (Associate Professor Lito Quirino)
ID Code:	114989
Year Published:	2016
Funding Support:	Australian Research Council (FT100100213)
Web of Science® Times Cited:	5
Deposited By:	Chemistry
Deposited On:	2017-03-03
Last Modified:	2018-03-16
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