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An isolate of Haemophilus haemolyticus produces a bacteriocin-like substance that inhibits the growth of nontypeable Haemophilus influenzae


Latham, RD and Gell, DA and Fairbairn, RL and Lyons, AB and Shukla, SD and Cho, KY and Jones, DA and Harkness, NM and Tristram, SG, An isolate of Haemophilus haemolyticus produces a bacteriocin-like substance that inhibits the growth of nontypeable Haemophilus influenzae, International Journal of Antimicrobial Agents, 49, (4) pp. 503-506. ISSN 0924-8579 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 Elsevier B.V.

DOI: doi:10.1016/j.ijantimicag.2016.12.010


Nontypeable Haemophilus influenzae (NTHi) frequently colonises the upper respiratory tract and is an important cause of respiratory infections. Resistance to antibiotics is an emerging trend in NTHi and alternative prevention or treatment strategies are required. Haemophilus haemolyticus is a common commensal occupying the same niche as NTHi and, if able to produce substances that inhibit NTHi growth, may have a role as a probiotic. In this study, ammonium sulphate extracts from broth culture of 100 H. haemolyticus isolates were tested for the presence of substances inhibitory to NTHi using a well diffusion assay. One isolate produced a substance that consistently inhibited the growth of NTHi. The substance was inactivated by protease enzymes and had a molecular size of ca. 30 kDa as determined by size exclusion chromatography. When the substance was tested against bacteria from eight Gram-negative and three Gram-positive genera, only Haemophilus spp. were inhibited. Quantitative PCR testing showed the substance to be different to 'haemocin', the previously described bacteriocin of H. influenzae type b. These molecular characteristics, together with narrow-spectrum activity, suggest the substance may be a novel bacteriocin, and there is potential for this H. haemolyticus isolate to function as a probiotic for reduction of colonisation and subsequent infection with NTHi.

Item Details

Item Type:Refereed Article
Keywords:haemophilus, haemolyticus, influenzae, probiotic, bacteriocin
Research Division:Biomedical and Clinical Sciences
Research Group:Medical microbiology
Research Field:Medical bacteriology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Latham, RD (Mr Roger Latham)
UTAS Author:Gell, DA (Dr David Gell)
UTAS Author:Fairbairn, RL (Mr Rory Fairbairn)
UTAS Author:Lyons, AB (Associate Professor Bruce Lyons)
UTAS Author:Shukla, SD (Mr Shakti Shukla)
UTAS Author:Cho, KY (Ms Kum Yin Cho)
UTAS Author:Tristram, SG (Dr Stephen Tristram)
ID Code:114980
Year Published:2017
Web of Science® Times Cited:14
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2017-03-03
Last Modified:2022-08-26

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