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Sex reversal of brook trout (Salvelinus fontinalis) by 17α-methyltestosterone exposure: a serial experimental approach to determine optimal timing and delivery regimes

Citation

Fatima, S and Adams, M and Wilkinson, R, Sex reversal of brook trout (Salvelinus fontinalis) by 17α-methyltestosterone exposure: a serial experimental approach to determine optimal timing and delivery regimes, Animal Reproduction Science, 175 pp. 39-47. ISSN 0378-4320 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier B.V.

DOI: doi:10.1016/j.anireprosci.2016.10.008

Abstract

Commercial culture of Brook trout (Salvelinus fontinalis) in Tasmania was partly abandoned due to sexual maturation of male fish early on during the estuarine rearing phase. Maturation adversely affects body mass, flesh quality and immunocompetency effectively. Sex reversal techniques such as the in-feed addition of a synthetic androgen have proven difficult to adapt in brook trout. An appropriate timing, duration and delivery vehicle for administration of 17α-methyltestosterone (MT) to produce phenotypic males (neomales) from genotypically female brook trout required further investigation. In this study, groups of brook trout eggs (n = 1000) maintained at 9.5 ± 0.15−10 ± 0.14 °C, were immersed in MT (400 μg L−1) for four hours on two alternate days (two immersions/group) staggered over a two week period surrounding the hatch of embryos (control groups excluded). The groups were then split and half received MT-supplemented feed for 60 days and the other a standard diet. Following an 11 month on-growing period sex phenotypes were determined by gross & histological gonad morphology. The highest proportion of male phenotypes (75%) was found in fish immersed six and four days pre-hatch and subsequently fed a normal diet. Fish fed a MT supplemented diet and immersed in MT showed significantly higher proportions of sterile fish. These data indicate that a pre-hatch immersion-only regime (4–6 days pre-hatch at 9.5 °C) should be pursued as a target for optimization studies to further refine the effective concentration and duration of exposure to MT for the successful production of neo-male brook trout.

Item Details

Item Type:Refereed Article
Keywords:brook trout, sex reversal, 17a-methyltestosterone, neomales
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal Developmental and Reproductive Biology
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Wild Caught
Objective Field:Fisheries - Recreational
Author:Fatima, S (Miss Shafaq Fatima)
Author:Adams, M (Dr Mark Adams)
Author:Wilkinson, R (Dr Ryan Wilkinson)
ID Code:114905
Year Published:2016
Web of Science® Times Cited:2
Deposited By:Centre for Fisheries and Aquaculture
Deposited On:2017-03-02
Last Modified:2017-10-31
Downloads:0

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