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Field-enhanced sample injection-micelle to solvent stacking in nonaqueous capillary electrophoresis
journal contribution
posted on 2023-05-19, 02:35 authored by Thang, LY, See, HH, Joselito QuirinoThe low conductivity of separation electrolytes employed in nonaqueous capillary electrophoresis (NACE) limits the use of on-line sample concentration or stacking by field enhancement. Herein, micelle-to-solvent stacking (MSS) was performed by the simple injection of a micellar solution plug prior to electrokinetic injection of sample prepared under field-enhanced stacking conditions (known as field-enhanced sample injection, FESI). The proposed approach allowed a 214–625-fold improvement in peak signals for targeted anticancer drugs (e.g., tamoxifen) and its major metabolites in NACE using 100% methanol-based separation electrolyte that comprised of 7.5 mM deoxycholic acid sodium salt, 15 mM acetic acid and 1 mM 18-crown-6. These improvements yielded tamoxifen and its metabolites with 2–5 times better stacking efficiency as compared to those obtained without micellar solution injection or FESI only. This is comparable to the results typically achieved when FESI is combined with isotachophoresis (electrokinetic supercharging). The FESI-MSS-NACE was tested for the measuring levels of target drugs in plasma. The analytical figures of merit are also reported.
Funding
Australian Research Council
History
Publication title
TalantaVolume
161Pagination
165-169ISSN
0039-9140Department/School
School of Natural SciencesPublisher
Elsevier BVPlace of publication
NetherlandsRights statement
Copyright 2016 Elsevier B.V.Repository Status
- Restricted