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Field-enhanced sample injection-micelle to solvent stacking in nonaqueous capillary electrophoresis


Thang, LY and See, HH and Quirino, JP, Field-enhanced sample injection-micelle to solvent stacking in nonaqueous capillary electrophoresis, Talanta, 161 pp. 165-169. ISSN 0039-9140 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Elsevier B.V.

DOI: doi:10.1016/j.talanta.2016.08.054


The low conductivity of separation electrolytes employed in nonaqueous capillary electrophoresis (NACE) limits the use of on-line sample concentration or stacking by field enhancement. Herein, micelle-to-solvent stacking (MSS) was performed by the simple injection of a micellar solution plug prior to electrokinetic injection of sample prepared under field-enhanced stacking conditions (known as field-enhanced sample injection, FESI). The proposed approach allowed a 214625-fold improvement in peak signals for targeted anticancer drugs (e.g., tamoxifen) and its major metabolites in NACE using 100% methanol-based separation electrolyte that comprised of 7.5 mM deoxycholic acid sodium salt, 15 mM acetic acid and 1 mM 18-crown-6. These improvements yielded tamoxifen and its metabolites with 25 times better stacking efficiency as compared to those obtained without micellar solution injection or FESI only. This is comparable to the results typically achieved when FESI is combined with isotachophoresis (electrokinetic supercharging). The FESI-MSS-NACE was tested for the measuring levels of target drugs in plasma. The analytical figures of merit are also reported.

Item Details

Item Type:Refereed Article
Keywords:Field enhanced sample injection; Micelle to solvent stacking; Nonaqueous capillary electrophoresis; Plasma; Tamoxifen
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:114839
Year Published:2016
Funding Support:Australian Research Council (FT100100213)
Web of Science® Times Cited:15
Deposited By:Chemistry
Deposited On:2017-02-28
Last Modified:2022-08-22

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