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Simultaneous determination of creatinine and acetate by capillary electrophoresis with contactless conductivity detector as a feasible approach for urinary tract infection diagnosis

Citation

Grochocki, W and Markuszewski, MJ and Quirino, JP, Simultaneous determination of creatinine and acetate by capillary electrophoresis with contactless conductivity detector as a feasible approach for urinary tract infection diagnosis, Journal of Pharmaceutical and Biomedical Analysis, 137 pp. 178-181. ISSN 0731-7085 (2017) [Refereed Article]

Copyright Statement

Crown Copyright ę 2017

DOI: doi:10.1016/j.jpba.2017.01.032

Abstract

Urinary tract infection (UTI) is one of the most common bacterial infection in human but its diagnosis is difficult. Metabolomic studies with nuclear magnetic resonance of urine have shown that acetic acid/creatinine ratio may be used for early UTI diagnosis. Here, a method for simultaneous determination of acetate and creatinine by capillary zone electrophoresis with contactless conductivity detector was developed for the first time. The separation was with 40ámM MES and 20ámM l-histidine as a background solution. The total time of a single run, including capillary conditioning, was less than 12ámin. The method was successfully demonstrated for analysis of actual and fortified human urine samples after methanol dilution. Analytical figures of merit such as linearity, LOQ, and repeatability (intraday and interday) were studied.

Item Details

Item Type:Refereed Article
Keywords:analysis
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Grochocki, W (Mr Wojciech Grochocki)
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:114838
Year Published:2017
Funding Support:Australian Research Council (FT100100213)
Web of Science® Times Cited:1
Deposited By:Chemistry
Deposited On:2017-02-28
Last Modified:2017-09-07
Downloads:0

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