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Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy

Citation

Chong, KC and Thang, LY and Quirino, JP and See, HH, Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy, Journal of Chromatography A, 1485 pp. 142-146. ISSN 0021-9673 (2017) [Refereed Article]

Copyright Statement

Copyright 2017 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2017.01.012

Abstract

A portable microchip electrophoresis (MCE) coupled with on-chip contactless conductivity detection (C 4D) system was evaluated for the determination of vancomycin in human plasma. In order to enhance the detection sensitivity, a new online multi-stacking preconcentration technique based on field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in MCE-C 4D system equipped with a commercially available double T-junction glass chip. The cationic analytes from the two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample-loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte and a sensitivity enhancement factor (SEF) of up to 217 was achieved. The developed approach was subsequently implemented in the aqueous-based MCE, coupled to C 4D in order to monitor the targeted antibiotic (in this case, vancomycin) present in human plasma samples. The multi-stacking and analysis time for vancomycin were 50s and 250s respectively, with SEF of approximately 83 when compared to typical gated injection. The detection limit of the method for vancomycin was 1.2μg/mL, with intraday and interday repeatability RSDs of 2.6% and 4.3%, respectively. Recoveries in spiked human plasma were 99.0%-99.2%.

Item Details

Item Type:Refereed Article
Keywords:analysis, micelle-to-solvent stacking, field-enhance sample injection, microchip electrophoresis, vancomycin, contactless conductivity detection
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:114832
Year Published:2017
Funding Support:Australian Research Council (FT100100213)
Web of Science® Times Cited:1
Deposited By:Chemistry
Deposited On:2017-02-28
Last Modified:2017-11-15
Downloads:0

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