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Capillary electrophoretic focusing of covalently derivatized protein induced by surfactant
journal contribution
posted on 2023-05-19, 02:34 authored by Oukacine, F, Joselito Quirino, Mesbah, K, Taverna, MIn this communication, we present a very simple strategy to focus covalently derivatized proteins for high sensitivity CE analysis by LIF detection. We demonstrated that the covalently tagged protein can be focused just by adding SDS at a concentration above the CMC in the derivatized sample. Under specific injection conditions, SDS concentration below the CMC is also sufficient to induce the focusing of the tagged protein. This method allows the quantification and detection of the covalently tagged protein in a narrow zone with an efficiency approaching 220 000 plates/m. Very good linearity was obtained for the ubiquitin in a concentration range of 2-25 μM.
Funding
Australian Research Council
History
Publication title
ElectrophoresisVolume
37Issue
9Pagination
1151-1154ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley - V C H Verlag GmbH & Co. KGaAPlace of publication
GermanyRights statement
Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimRepository Status
- Restricted