eCite Digital Repository

Capillary electrophoretic focusing of covalently derivatized protein induced by surfactant

Citation

Oukacine, F and Quirino, JP and Mesbah, K and Taverna, M, Capillary electrophoretic focusing of covalently derivatized protein induced by surfactant, Electrophoresis, 37, (9) pp. 1151-1154. ISSN 0173-0835 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/elps.201600030

Abstract

In this communication, we present a very simple strategy to focus covalently derivatized proteins for high sensitivity CE analysis by LIF detection. We demonstrated that the covalently tagged protein can be focused just by adding SDS at a concentration above the CMC in the derivatized sample. Under specific injection conditions, SDS concentration below the CMC is also sufficient to induce the focusing of the tagged protein. This method allows the quantification and detection of the covalently tagged protein in a narrow zone with an efficiency approaching 220 000 plates/m. Very good linearity was obtained for the ubiquitin in a concentration range of 2-25 μM.

Item Details

Item Type:Refereed Article
Keywords:analysis
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:114827
Year Published:2016
Funding Support:Australian Research Council (FT100100213)
Web of Science® Times Cited:1
Deposited By:Chemistry
Deposited On:2017-02-28
Last Modified:2017-10-31
Downloads:0

Repository Staff Only: item control page