Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in methanol and the resulting sample solution was pressure injected after a micellar plug of SDS. Factors that affected the stacking such as SDS concentration, micelle, and sample plug length were examined. The sensitivity enhancement factor (peak height from stacking/peak height from typical injection of sample in BGE) was 15-22. The method detection limits with LLE were in the range of 5-10 ng/mL, which was lower than the established method (where the LLE extract was also prepared in methanol) with reported method detection limits of 25-40 ng/mL. The intraday and interday repeatability were in the range of 1.0-3.4% and 3.8-6.5%, respectively.

Item Type:	Refereed Article
Keywords:	analysis
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Expanding Knowledge
Objective Group:	Expanding knowledge
Objective Field:	Expanding knowledge in the chemical sciences
UTAS Author:	Thang, LY (Ms Lee Yien Thang)
UTAS Author:	See, HH (Dr Hong Heng See)
UTAS Author:	Quirino, JP (Associate Professor Lito Quirino)
ID Code:	114824
Year Published:	2016
Funding Support:	Australian Research Council (FT100100213)
Web of Science® Times Cited:	22
Deposited By:	Chemistry
Deposited On:	2017-02-28
Last Modified:	2017-10-31
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