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Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis

Citation

Thang, LY and See, HH and Quirino, JP, Determination of tamoxifen and its metabolites using micelle to solvent stacking in nonaqueous capillary electrophoresis, Electrophoresis, 37, (9) pp. 1166-1169. ISSN 0173-0835 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/elps.201600010

Abstract

Micelle to solvent stacking was implemented for the recently established NACE-C4D method to determine tamoxifen and its metabolites in standard samples and human plasma of breast cancer patients. For stacking, the standard samples and extract after liquid-liquid extraction (LLE) were prepared in methanol and the resulting sample solution was pressure injected after a micellar plug of SDS. Factors that affected the stacking such as SDS concentration, micelle, and sample plug length were examined. The sensitivity enhancement factor (peak height from stacking/peak height from typical injection of sample in BGE) was 15-22. The method detection limits with LLE were in the range of 5-10 ng/mL, which was lower than the established method (where the LLE extract was also prepared in methanol) with reported method detection limits of 25-40 ng/mL. The intraday and interday repeatability were in the range of 1.0-3.4% and 3.8-6.5%, respectively.

Item Details

Item Type:Refereed Article
Keywords:analysis
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Thang, LY (Ms Lee Yien Thang)
Author:See, HH (Dr Hong Heng See)
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:114824
Year Published:2016
Funding Support:Australian Research Council (FT100100213)
Web of Science® Times Cited:5
Deposited By:Chemistry
Deposited On:2017-02-28
Last Modified:2017-10-31
Downloads:0

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