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Simultaneous electrophoretic concentration and separation of herbicides in beer prior to stacking capillary electrophoresis UV and liquid chromatography-mass spectrometry

Citation

Wuethrich, A and Haddad, PR and Quirino, JP, Simultaneous electrophoretic concentration and separation of herbicides in beer prior to stacking capillary electrophoresis UV and liquid chromatography-mass spectrometry, Electrophoresis, 37, (9) pp. 1122-1128. ISSN 0173-0835 (2016) [Refereed Article]

Copyright Statement

Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

DOI: doi:10.1002/elps.201600050

Abstract

Simultaneous electrophoretic concentration and separation (SECS) was used as a simple and environmental friendly sample preparation strategy for herbicides in beer samples. An electric field was used to facilitate the separation and concentration of the analytes based on their charge from a 20 mL sample of diluted beer into two separate 20 μL aliquots of an acceptor electrolyte housed inside a micropipette. The anionic organophosphonate and cationic quaternary ammonium herbicides were concentrated in the anodic and cathodic pipette, respectively. Under optimized conditions, SECS was completed in 30 min at an applied voltage of 150 V, which provided analyte concentration factors of up to 90. After sample preparation, the SECS concentrate of cationic and anionic herbicides was analyzed by stacking CE with UV detection and also by LC-MS, respectively. The method detection limit for the diluted and undiluted sample was as low as 3 and 15 ng/mL, respectively. The method was linear over two orders of concentration with repeatability and intermediate precision of better than 5.8 and 7.0%RSD, respectively. Accuracy values were between 91.0-115.1%.

Item Details

Item Type:Refereed Article
Keywords:sample preparation
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Wuethrich, A (Mr Alain Wuethrich)
Author:Haddad, PR (Professor Paul Haddad)
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:114820
Year Published:2016
Funding Support:Australian Research Council (FT100100213)
Web of Science® Times Cited:6
Deposited By:Chemistry
Deposited On:2017-02-28
Last Modified:2017-10-31
Downloads:0

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