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Simultaneous electrophoretic concentration and separation of herbicides in beer prior to stacking capillary electrophoresis UV and liquid chromatography-mass spectrometry
Citation
Wuethrich, A and Haddad, PR and Quirino, JP, Simultaneous electrophoretic concentration and separation of herbicides in beer prior to stacking capillary electrophoresis UV and liquid chromatography-mass spectrometry, Electrophoresis, 37, (9) pp. 1122-1128. ISSN 0173-0835 (2016) [Refereed Article]
Copyright Statement
Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
DOI: doi:10.1002/elps.201600050
Abstract
Simultaneous electrophoretic concentration and separation (SECS) was used as a simple and environmental friendly sample preparation strategy for herbicides in beer samples. An electric field was used to facilitate the separation and concentration of the analytes based on their charge from a 20 mL sample of diluted beer into two separate 20 μL aliquots of an acceptor electrolyte housed inside a micropipette. The anionic organophosphonate and cationic quaternary ammonium herbicides were concentrated in the anodic and cathodic pipette, respectively. Under optimized conditions, SECS was completed in 30 min at an applied voltage of 150 V, which provided analyte concentration factors of up to 90. After sample preparation, the SECS concentrate of cationic and anionic herbicides was analyzed by stacking CE with UV detection and also by LC-MS, respectively. The method detection limit for the diluted and undiluted sample was as low as 3 and 15 ng/mL, respectively. The method was linear over two orders of concentration with repeatability and intermediate precision of better than 5.8 and 7.0%RSD, respectively. Accuracy values were between 91.0-115.1%.
Item Details
Item Type: | Refereed Article |
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Keywords: | sample preparation |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Wuethrich, A (Mr Alain Wuethrich) |
UTAS Author: | Haddad, PR (Professor Paul Haddad) |
UTAS Author: | Quirino, JP (Associate Professor Lito Quirino) |
ID Code: | 114820 |
Year Published: | 2016 |
Funding Support: | Australian Research Council (FT100100213) |
Web of Science® Times Cited: | 13 |
Deposited By: | Chemistry |
Deposited On: | 2017-02-28 |
Last Modified: | 2022-08-22 |
Downloads: | 0 |
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