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Simultaneous electrophoretic concentration and separation of herbicides in beer prior to stacking capillary electrophoresis UV and liquid chromatography-mass spectrometry
journal contribution
posted on 2023-05-19, 02:33 authored by Wuethrich, A, Paul HaddadPaul Haddad, Joselito QuirinoSimultaneous electrophoretic concentration and separation (SECS) was used as a simple and environmental friendly sample preparation strategy for herbicides in beer samples. An electric field was used to facilitate the separation and concentration of the analytes based on their charge from a 20 mL sample of diluted beer into two separate 20 μL aliquots of an acceptor electrolyte housed inside a micropipette. The anionic organophosphonate and cationic quaternary ammonium herbicides were concentrated in the anodic and cathodic pipette, respectively. Under optimized conditions, SECS was completed in 30 min at an applied voltage of 150 V, which provided analyte concentration factors of up to 90. After sample preparation, the SECS concentrate of cationic and anionic herbicides was analyzed by stacking CE with UV detection and also by LC-MS, respectively. The method detection limit for the diluted and undiluted sample was as low as 3 and 15 ng/mL, respectively. The method was linear over two orders of concentration with repeatability and intermediate precision of better than 5.8 and 7.0%RSD, respectively. Accuracy values were between 91.0-115.1%.
Funding
Australian Research Council
History
Publication title
ElectrophoresisVolume
37Issue
9Pagination
1122-1128ISSN
0173-0835Department/School
School of Natural SciencesPublisher
Wiley - V C H Verlag GmbH & Co. KGaAPlace of publication
GermanyRights statement
Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimRepository Status
- Restricted