eCite Digital Repository

The NEAT Domain-Containing Proteins of Clostridium perfringens Bind Heme

Citation

Choo, JM and Cheung, JK and Wisniewski, JA and Steer, DL and Bulach, DM and Hiscox, TJ and Chakravorty, A and Smith, AI and Gell, DA and Rood, JI and Award, MA, The NEAT Domain-Containing Proteins of Clostridium perfringens Bind Heme, PloS one, 11, (9) Article e0162981. ISSN 1932-6203 (2016) [Refereed Article]


Preview
PDF
3Mb
  

Copyright Statement

Copyright 2016 Choo et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0162981

Abstract

The ability of a pathogenic bacterium to scavenge iron from its host is important for its growth and survival during an infection. Our studies on C. perfringens gas gangrene strain JIR325, a derivative of strain 13, showed that it is capable of utilizing both human hemoglobin and ferric chloride, but not human holo-transferrin, as an iron source for in vitro growth. Analysis of the C. perfringens strain 13 genome sequence identified a putative heme acquisition system encoded by an iron-regulated surface gene region that we have named the Cht (Clostridium perfringens heme transport) locus. This locus comprises eight genes that are co-transcribed and includes genes that encode NEAT domain-containing proteins (ChtD and ChtE) and a putative sortase (Srt). The ChtD, ChtE and Srt proteins were shown to be expressed in JIR325 cells grown under iron-limited conditions and were localized to the cell envelope. Moreover, the NEAT proteins, ChtD and ChtE, were found to bind heme. Both chtDE and srt mutants were constructed, but these mutants were not defective in hemoglobin or ferric chloride utilization. They were, however, attenuated for virulence when tested in a mouse myonecrosis model, although the virulence phenotype could not be restored via complementation and, as is common with such systems, secondary mutations were identified in these strains. In summary, this study provides evidence for the functional redundancies that occur in the heme transport pathways of this life threatening pathogen.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Medical Microbiology
Research Field:Medical Bacteriology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Gell, DA (Dr David Gell)
ID Code:114812
Year Published:2016
Deposited By:Medicine (Discipline)
Deposited On:2017-02-28
Last Modified:2017-03-21
Downloads:38 View Download Statistics

Repository Staff Only: item control page